Zobrazeno 1 - 2
of 2
pro vyhledávání: '"Carol Ann Gallegos"'
Autor:
Diane E. Cabelli, Robert A. Hallewell, Pandora Lee, Noel M. Fong, Carol Ann Gallegos, Karin C. Imlay, Elizabeth D. Getzoff, Lawrence S Cousens, Guy T. Mullenbach, John A. Tainer, Patricia Tekamp-Olson
Publikováno v:
Biochemical and Biophysical Research Communications. 181:474-480
Human CuZn superoxide dismutase (HSOD) has two free cysteines: a buried cysteine (Cys6) located in a beta-strand, and a solvent accessible cysteine (Cys111) located in a loop region. The highly homologous bovine enzyme (BSOD) has a single buried Cys6
Autor:
Mickey S. Urdea, Jeffrey R. Shuster, Lailing Ku, Lawrence S. Cousens, Ray Sanchez-Pescador, Michelle M. Stempien, Carol Ann Gallegos, Alice Taylor, Patricia Tekamp-Olson
Publikováno v:
Gene. 61:265-275
Human proinsulin (PI) has been expressed to a high level (100 mg/liter) as a human Superoxide dismutase-PI fusion protein in the yeast, Saccharomyces cerevisiae . At the junction of the two proteins is a methionine residue, allowing PI to be released