Biomarkers of collagen synthesis predict progression in the PROFILE idiopathic pulmonary fibrosis cohort

Autor: Louise A. Organ, Anne-Marie R. Duggan, Eunice Oballa, Sarah C. Taggart, Juliet K. Simpson, Arthur R. Kang’ombe, Rebecca Braybrooke, Philip L. Molyneaux, Bernard North, Yakshitha Karkera, Diana J. Leeming, Morten A. Karsdal, Carmel B. Nanthakumar, William A. Fahy, Richard P. Marshall, R. Gisli Jenkins, Toby M. Maher

Publikováno v: Respiratory Research, Vol 20, Iss 1, Pp 1-10 (2019)

Abstract Idiopathic pulmonary fibrosis (IPF) is characterised by excessive extracellular matrix (ECM) deposition and remodelling. Measuring this activity provides an opportunity to develop tools capable of identifying individuals at-risk of progressi

Externí odkaz: https://doaj.org/article/bf04a004c4ea420686e599942cce6684

The mTORC1/4E-BP1 axis represents a critical signaling node during fibrogenesis

Autor: Hannah V. Woodcock, Jessica D. Eley, Delphine Guillotin, Manuela Platé, Carmel B. Nanthakumar, Matteo Martufi, Simon Peace, Gerard Joberty, Daniel Poeckel, Robert B. Good, Adam R. Taylor, Nico Zinn, Matthew Redding, Ellen J. Forty, Robert E. Hynds, Charles Swanton, Morten Karsdal, Toby M. Maher, Andrew Fisher, Giovanna Bergamini, Richard P. Marshall, Andy D. Blanchard, Paul F. Mercer, Rachel C. Chambers

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019)

The PI3K/Akt/mTOR pathway has been previously implicated in fibrosis and a pan-PI3K/mTOR inhibitor is currently under clinical evaluation for the treatment of IPF. Here the authors show that the mTORC1/4E-BP1 axis is critical for TGF-β1-induced fibr

Externí odkaz: https://doaj.org/article/c3c84960b35344d79ec192d4954f0d3a

Single-Step, High-Efficiency CRISPR-Cas9 Genome Editing in Primary Human Disease-Derived Fibroblasts

Autor: Klio Maratou, Radu Rapiteanu, Eleni Patili, Matteo Martufi, Tobias Schmidt, Robert B. Good, Maria New, Carmel B. Nanthakumar, Andy Blanchard, Joanna Betts, Ketil Tvermosegaard

Publikováno v: The CRISPR Journal

Genome editing is a tool that has many applications, including the validation of potential drug targets. However, performing genome editing in low-passage primary human cells with the greatest physiological relevance is notoriously difficult. High ed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4e2a1ce9d2fe6fe8ab8cf1c4e1d9a68
https://doi.org/10.1089/crispr.2018.0047

Prolonged Scar-in-a-Jar:an in vitro screening tool for anti-fibrotic therapies using biomarkers of extracellular matrix synthesis

Autor: Sarah Brockbank, Grith Lykke Sørensen, Federica Genovese, Diana Julie Leeming, Jannie M.B. Sand, Henrik Jessen, Vikki J. Barrett, Sarah Rønnow, Morten A. Karsdal, Robert B. Good, Simon Cruwys, Carmel B. Nanthakumar, Rand Qais Dabbagh

Publikováno v: Rønnow, S R, Dabbagh, R Q, Genovese, F, Nanthakumar, C B, Barrett, V J, Good, R B, Brockbank, S, Cruwys, S, Jessen, H, Sorensen, G L, Karsdal, M A, Leeming, D J & Sand, J M B 2020, ' Prolonged Scar-in-a-Jar : an in vitro screening tool for anti-fibrotic therapies using biomarkers of extracellular matrix synthesis ', Respiratory Research, vol. 21, 108 . https://doi.org/10.1186/s12931-020-01369-1
Respiratory Research, Vol 21, Iss 1, Pp 1-14 (2020)
Respiratory Research

Background Idiopathic pulmonary fibrosis (IPF) is a rapidly progressing disease with challenging management. To find novel effective therapies, better preclinical models are needed for the screening of anti-fibrotic compounds. Activated fibroblasts d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd4bca4d89576887e6d121d1b78582a7
https://findresearcher.sdu.dk:8443/ws/files/169994955/Open_Access_Version.pdf

c-Rel orchestrates energy-dependent epithelial and macrophage reprogramming in fibrosis

Autor: Johannes L Zakrzewski, Ben S. Barksby, Jeremy French, Luc Schoonjans, Amy L Collins, Jelena Mann, Matthias Trost, Stuart Robinson, Ulf Klein, Morten A. Karsdal, Hannah L Paish, Amber Knox, Peter Carmeliet, Lee A. Borthwick, Andrew D Blanchard, Git Chung, Rainie Cameron, Neil S. Sheerin, Laure-Anne Teuwen, Ingmar Mederacke, Lucy M Gee, Colin D.A. Brown, Carmel B. Nanthakumar, Thomas G. Bird, Jack Leslie, Sandra Murphy, Robert F. Schwabe, Fiona Oakley, Marina García Macia, Xin Xu, Andrew J. Fisher, Derek A. Mann, Derek Manas, Rachel A. Burgoyne, William J Reilly, Steven A. White, Charlotte Bragg, Saimir Luli, Gourab Sen, Marco Y W Zaki, Colin Nixon, Julie C. Worrell

Publikováno v: Nature Metabolism
Leslie, J, Macia, M G, Luli, S, Worrell, J C, Reilly, W J, Paish, H L, Knox, A, Barksby, B S, Gee, L M, Zaki, M Y W, Collins, A L, Burgoyne, R A, Cameron, R, Bragg, C, Xu, X, Chung, G W, Brown, C D A, Blanchard, A D, Nanthakumar, C B, Karsdal, M, Robinson, S M, Manas, D M, Sen, G, French, J, White, S A, Murphy, S, Trost, M, Zakrzewski, J L, Klein, U, Schwabe, R F, Mederacke, I, Nixon, C, Bird, T, Teuwen, L-A, Schoonjans, L, Carmeliet, P, Mann, J, Fisher, A J, Sheerin, N S, Borthwick, L A, Mann, D A & Oakley, F 2020, ' c-Rel orchestrates energy-dependent epithelial and macrophage reprogramming in fibrosis ', Nature Metabolism, vol. 2, no. 11, pp. 1350-1367 . https://doi.org/10.1038/s42255-020-00306-2
Nat Metab
Digital.CSIC. Repositorio Institucional del CSIC
instname

Fibrosis is a common pathological feature of chronic disease. Deletion of the NF-κB subunit c-Rel limits fibrosis in multiple organs, although the mechanistic nature of this protection is unresolved. Using cell-specific gene-targeting manipulations

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a29e2b56012d84a4113925afd0985a4b