Manual and automated synthesis of modified oligonucleotides on core–shell solid supports

Autor: Luis M. Menéndez-Méndez, Carme Fàbrega, Anna Aviñó, Ramon Eritja, Yoon-Sik Lee, Yogesh S. Sanghvi, Susana Fernández, Miguel Ferrero

Publikováno v: Results in Chemistry, Vol 12, Iss , Pp 101930- (2024)

A critical step in the manufacturing of oligonucleotides is the selection of ideal solid supports. Herein, we evaluate a hybrid core–shell solid support made of polystyrene and polyethyleneglycol, for the preparation of oligonucleotides using phosp

Externí odkaz: https://doaj.org/article/5d46ba76923f474c957e8a91dcfa08de

Synthesis of 2′‑O‑Methyl/2′‑O‑MOE-L-Nucleoside Derivatives and Their Applications: Preparation of G‑Quadruplexes, Their Characterization, and Stability Studies

Autor: Virginia Martín-Nieves, Luis Miguel Menéndez-Méndez, Carme Fàbrega, Susana Fernández, Yogesh S. Sanghvi, Miguel Ferrero, Ramon Eritja

Publikováno v: ACS Omega, Vol 8, Iss 47, Pp 44893-44904 (2023)

Externí odkaz: https://doaj.org/article/b7a56be495fe4d44af169a1bde2d8f4c

Parallel G-quadruplex Structures Increase Cellular Uptake and Cytotoxicity of 5-Fluoro-2′-deoxyuridine Oligomers in 5-Fluorouracil Resistant Cells

Autor: Anna Clua, Carme Fàbrega, Jesús García-Chica, Santiago Grijalvo, Ramon Eritja

Publikováno v: Molecules, Vol 26, Iss 6, p 1741 (2021)

Fluoropyrimidines, such as 5-fluorouracil (5-FU) and related prodrugs have been considered first-line chemotherapy agents for the treatment of colorectal cancer. However, poor specificity and tumor cell resistance remain major limiting bottlenecks. G

Externí odkaz: https://doaj.org/article/961e53e408164cf6b10be2d14af57cc6

Development of a Novel Fluorescence Assay Based on the Use of the Thrombin-Binding Aptamer for the Detection of O6-Alkylguanine-DNA Alkyltransferase Activity

Autor: Maria Tintoré, Anna Aviñó, Federico M. Ruiz, Ramón Eritja, Carme Fàbrega

Publikováno v: Journal of Nucleic Acids, Vol 2010 (2010)

Human O6-alkylguanine-DNA alkyltransferase (hAGT) is a DNA repair protein that reverses the effects of alkylating agents by removing DNA adducts from the O6 position of guanine. Here, we developed a real-time fluorescence hAGT activity assay that is

Externí odkaz: https://doaj.org/article/03b526e5b7f548e6974686e91bf09380

Evaluation of Floxuridine Oligonucleotide Conjugates Carrying Potential Enhancers of Cellular Uptake

Autor: Ramon Eritja, María José Bleda, Anna Aviñó, Carme Fàbrega, Anna Clua

Publikováno v: International Journal of Molecular Sciences
Volume 22
Issue 11
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Digital.CSIC. Repositorio Institucional del CSIC
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International Journal of Molecular Sciences, Vol 22, Iss 5678, p 5678 (2021)

Conjugation of small molecules such as lipids or receptor ligands to anti-cancer drugs has been used to improve their pharmacological properties. In this work, we studied the biological effects of several small-molecule enhancers into a short oligonu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e5dd5cd58a06ff454fb1560afbe0f15
http://europepmc.org/articles/PMC8199350