Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Carlos Spears"'
Publikováno v:
Journal of Biological Chemistry. 252:6889-6894
The phage DNA-directed synthesis of beta-galactosidase has been examined in a system containing the following purified Escherichia coli factors: RNA polymerase; cyclic AMP receptor protein; N10-formyltetrahydrofolate Met-tRNAf transformylase; initiat
Publikováno v:
Archives of Biochemistry and Biophysics. 162:578-584
The factors present in the NH 4 Cl wash of ribosomes that are required for the DNA directed in vitro synthesis of β-galactosidase have been examined in a system dependent on washed Escherichia coli ribosomes, the NH 4 Cl wash of ribosomes, and a 200
Autor:
Hsiang-Fu Kung, Frederick Chu, Herbert Weissbach, Barnet Eskin, Nathan Brot, Carlos Spears, Benjamin V. Treadwell, Paul Caldwell
Publikováno v:
Archives of Biochemistry and Biophysics. 187:457-463
A crude messenger-RNA preparation capable of directing the synthesis of the α-peptide of β-galactosidase, ribosomal proteins L12 and L10, and elongation factor Tu (EF-Tu) has been obtained from a β-galactosidase overproducing strain, Escherichia c
Publikováno v:
Archives of Biochemistry and Biophysics. 160:168-174
With the use of rifampicin to stop the initiation of RNA synthesis, the in vitro synthesis of β -galactosidase has been carried out in two separate stages, transcription and translation. In this system it has been possible to examine whether substan
Publikováno v:
Archives of Biochemistry and Biophysics. 159:880-884
Free and membrane bound polysomes were prepared from 8-day-old chick embryos. Both polysome preparations were equally active in protein synthesis but procollagen-collagen synthesis was carried out exclusively by the membrane bound polysomes. The coll
Autor:
Carlos Spears, Benjamin V. Treadwell, Betty Redfield, Barnet Eskin, Hsiang-Fu Kung, Herbert Weissbach
Publikováno v:
Archives of Biochemistry and Biophysics. 189:531-534
Two forms of initiation factor 2, (IF-2α, Mr, 118,000 and IF-2β, Mr 90,000) have been isolated from Escherichia coli extracts and tested for their ability to support β-galactosidase synthesis in a phage DNA-directed in vitro protein synthesis syst
Publikováno v:
Archives of Biochemistry and Biophysics. 158:249-256
The effects of supplementation of growth medium with high concentrations of methionine (5 m m ) and/or vitamin B12 (10 n m ) on the activities of five enzymes of the methionine regulon were measured in wild-type Escherichia coli K12, a metJ prototrop
Publikováno v:
Archives of Biochemistry and Biophysics. 150:23-31
Two of the enzymes involved in the terminal steps of methionine biosynthesis, N 5,10 -methylene-H 4 -folate reductase and the non-B 12 transmethylase, are repressed in a noncoordinate manner by both vitamin B 12 and methionine. The repressions due to
Publikováno v:
Journal of Biological Chemistry. 248:5012-5015
The DNA-directed in vitro synthesis of β-galactosidase has been examined in a system dependent on Escherichia coli ribosomes, a salt wash of the ribosomes, and a 200,000 x g supernatant fraction. The ribosomes can be replaced by 30 S and 50 S riboso
Publikováno v:
Archives of Biochemistry and Biophysics. 142:231-237
In the present report some aspects of vitamin B 12 metabolism in HeLa cells and other tissue culture lines have been investigated. Mangum et al . (1) previously reported the presence of higher levels of N 5 -methyl- H 4 -folate-homocysteine transmeth