Výsledky vyhledávání - "Carlo Schmelzer"

RNA editing of a group II intron in Oenothera as a prerequisite for splicing

Autor: Mario Mörl, Carlo Schmelzer, Bernd Wissinger, G.V. Börner, Axel Brennicke

Publikováno v: Molecular and General Genetics MGG. 246:739-744

The trans-splicing group II intron c/d in the Oenothera mitochondrial nad1 gene is modified by RNA editing in domain 6. This C-to-U conversion generates the typical domain 6 structure, which prompted us to speculate that this RNA editing event might

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0115836049d3f6dbbb1fd44d543d394c
https://doi.org/10.1007/bf00290721

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Overexpression of DEAD box protein pMSS116 promotes ATP-dependent splicing of a yeast group II intronin vitro

Autor: Carlo Schmelzer, Isabell Niemer, G. Valentin Boörner

Publikováno v: Nucleic Acids Research. 23:2966-2972

The group II intron bI1, the first intron of the mitochondrial cytochrome b gene in yeast is self-splicing in vitro. Genetic evidence suggests that trans-acting factors are required for in vivo splicing of this intron. In accordance with these findin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30f5a0e8cd9b815e971d43fed76f02db
https://doi.org/10.1093/nar/23.15.2966

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Self-splicing of a Podospora anserina Group IIA Intron in vitro

Autor: Udo Schmidt, Carlo Schmelzer, Ralf Sägebarth, Ulf Stahl

Publikováno v: Journal of Molecular Biology. 231:559-568

A shortened derivative of the group IIA intron from the mitochondrial cytochrome-c-oxidase subunit I gene (COI II) of the ascomycete Podospora anserina can undergo self-splicing in vitro. When compared to self-splicing group IIB introns from yeast mi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e31dd063cb14ad80aeec59058d3a78ef
https://doi.org/10.1006/jmbi.1993.1309

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Restoration of the self-splicing activity of a defective group II intron by a small trans-acting RNA

Autor: Mathias Suchy, Carlo Schmelzer

Publikováno v: Journal of Molecular Biology. 222:179-187

The yeast mitochondrial group II intron bI1 is self-splicing in vitro. We have introduced a deletion of hairpin C1 within the structural domain 1 that abolishes catalytic activity of the intron in the normal splicing reaction in cis, but does less se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d489108623b94f215455638f092b73eb
https://doi.org/10.1016/0022-2836(91)90204-j

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Effect of deletions at structural domains of group II intron bI1 on self-splicing in vitro

Autor: Carlo Schmelzer, Jürgen Bachl

Publikováno v: Journal of Molecular Biology. 212:113-125

Some group II introns can undergo a protein-independent splicing reaction with the basic reaction pathway similar to nuclear pre-mRNA splicing and the catalytic functions of some of the structural components have been determined. To identify further

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::429a43c629611e0058d6b46677ce38fd
https://doi.org/10.1016/0022-2836(90)90308-9

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Integration of group II intron bl1 into a foreign RNA by reversal of the self-splicing reaction in vitro

Autor: Carlo Schmelzer, Mario Mörl

Publikováno v: Cell. 60:629-636

Group II intron bl 1, the first intron of the COB gene in the mitochondria of S. cerevisiae, is able to self-splice in vitro with the basic pathway similar to nuclear pre-mRNA splicing. We show that incubation of the intron lariat with ligated exons

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4978d0740b5049717d0d0964c2222905
https://doi.org/10.1016/0092-8674(90)90666-3

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New reactions catalyzed by a group II intron ribozyme with RNA and DNA substrates

Autor: Carlo Schmelzer, Isabell Niemer, Mario Mörl

Publikováno v: Cell. 70(5)

Here we describe three novel reactions of the self-splicing group II intron bI1 (the first intron of the COB gene of yeast mitochondria) demonstrating its catalytic versatility: reversal of the first step of the self-splicing reaction catalyzed by a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc6c54523b4af352e1bc1ed610e45692
https://pubmed.ncbi.nlm.nih.gov/1381286

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Structural requirements for selection of 5'- and 3' splice sites of group II introns

Autor: Carlo Schmelzer, Mario Mörl, Isabell Niemer, C Wallasch

Publikováno v: Nucleic acids research. 19(12)

The group II intron bl1 in the gene for apocytochrome b in yeast mitochondrial DNA (COB) is self-splicing in vitro. It could recently be shown that self-splicing of this intron is fully reversible in vitro. In addition, intron integration is not rest

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3f7120540baf814886f7c0c97293956
https://pubmed.ncbi.nlm.nih.gov/2062646

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Group II intron RNA-catalyzed recombination of RNA in vitro

Autor: Mario Mörl, Carlo Schmelzer

Publikováno v: Nucleic acids research. 18(22)

We report the first evidence for a novel reaction mediated by the self-splicing yeast mitochondrial group II intron bl1; the site-specific recombination of RNA molecules in vitro. Upon incubation of the intron lariat with two different RNAs, each har

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17399cee50aaf37747ee8b4828f66800
https://pubmed.ncbi.nlm.nih.gov/1701241

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