Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Carles de Mas"'
Autor:
Glòria González, Gregorio Álvaro, Max Cárdenas-Fernández, Glòria Carminal, Carles de Mas, Antoni Casablancas, Josep López-Santín, Carmen López, Maria Dolors Benaiges
Publikováno v:
Recercat. Dipósit de la Recerca de Catalunya
instname
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Electronic Journal of Biotechnology, Volume: 16, Issue: 3, Pages: 4-4, Published: 15 MAY 2013
Electronic Journal of Biotechnology v.16 n.3 2013
SciELO Chile
CONICYT Chile
instacron:CONICYT
instname
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Electronic Journal of Biotechnology, Volume: 16, Issue: 3, Pages: 4-4, Published: 15 MAY 2013
Electronic Journal of Biotechnology v.16 n.3 2013
SciELO Chile
CONICYT Chile
instacron:CONICYT
Background: New enzymes for biotransformations can be obtained by different approaches including directed mutagenesis and in vitro evolution. These mutants have to be efficiently produced for laboratory research on bioreactions as well as for process
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e48deded3e5be53b883e35905f3952a0
http://hdl.handle.net/2072/435735
http://hdl.handle.net/2072/435735
Publikováno v:
Biotechnology and Bioengineering. 113:772-782
An overall model describing the dynamic behavior of fed-batch E. coli processes for protein production has been built, calibrated and validated. Using a macroscopic approach, the model consists of three interconnected blocks allowing simulation of bi
Publikováno v:
Biochemical Engineering Journal. 91:210-219
An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β- d -tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 Δ glyA [pQEαβrham] [pREP4] p
Autor:
Carles de Mas, Martina Pasini, Gloria Caminal, Alfred Fernández-Castané, Pau Ferrer, Alfonso Jaramillo
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
instname
instname
The over-expression of proteins in recombinant host cells often requires a significant amount of resources causing an increase in the metabolic load for the host. This results in a variety of physiological responses leading to altered growth paramete
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe5ea3b0062e6e837f3b59f3d26e12e8
http://wrap.warwick.ac.uk/71872/7/WRAP_0380721-lf-270815-5f36aa53-843d-4dec-9d2a-9652b3c4ebaa.pdf
http://wrap.warwick.ac.uk/71872/7/WRAP_0380721-lf-270815-5f36aa53-843d-4dec-9d2a-9652b3c4ebaa.pdf
Publikováno v:
Biochemical Engineering Journal. 55:82-91
A semi-empirical mathematical model for recombinant rhamnulose 1-phosphate aldolase (RhuA) production in high cell density cultures of Escherichia coli has been developed. An unstructured and non-segregated approach allowed to identify the inducer–
Alternative production process strategies in E. coli improving protein quality and downstream yields
Autor:
Josep López-Santín, Gregorio Álvaro, Glòria González, Jaume Pinsach, Jordi Ruiz, Carles de Mas, David Resina
Publikováno v:
Process Biochemistry. 44:1039-1045
Process strategies for production of recombinant rhamnulose 1-phosphate aldolase (RhuA) in Escherichia coli were found to have an important impact on downstream processing when preparing the enzyme for its use as immobilized biocatalyst. First, a con
Publikováno v:
Enzyme and Microbial Technology. 43:507-512
The influence of proteolysis over recombinant protein quality has been studied using rhamnulose 1-phosphate aldolase (RhuA) production as case example. Progressive induction by means of continuous isopropyl-β- d -thiogalactopyranoside (IPTG) dosage
Publikováno v:
Biochemical Engineering Journal. 41:181-187
Different induction strategies for fed-batch recombinant protein production under the control of the strong T5 promoter in Escherichia coli have been investigated. Since the production of recombinant rhamnulose 1-phosphate aldolase is growth-related,
Publikováno v:
Journal of Chemical Technology & Biotechnology. 44:275-288
A kinetic model for enzymatic hydrolysis of pretreated wheat straw able to describe the reaction process considering only macroscopic properties of enzyme and substrate would be useful in reactor design and scale-up. In this study Michaelis-Menten eq
Publikováno v:
Biotechnology and bioengineering. 113(4)
An overall model describing the dynamic behavior of fed-batch E. coli processes for protein production has been built, calibrated and validated. Using a macroscopic approach, the model consists of three interconnected blocks allowing simulation of bi