A tight tunable range for Ni(II) sensing and buffering in cells

Autor: Peter T. Chivers, Lars-Olof Pålsson, Carl J. Patterson, Ehmke Pohl, Andrew W. Foster, Ian Cummins, Rafael Pernil, Andrew J. P. Scott, Nigel J. Robinson, Robert Pal

Publikováno v: Nature chemical biology, 2017, Vol.13(4), pp.409-414 [Peer Reviewed Journal]
ResearcherID
Nature chemical biology

The metal affinities of metal-sensing transcriptional regulators co-vary with cellular metal concentrations over more than 12 orders of magnitude. To understand the cause of this relationship, we determined the structure of the Ni(II) sensor InrS and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ad98237b5f1e466e32a72cd3f732fc3
https://doi.org/10.1038/nchembio.2310

Cytosolic Ni(II) Sensor in Cyanobacterium

Autor: Corinna R. Hess, Andrew W. Foster, Carl J. Patterson, Rafael Pernil, Nigel J. Robinson

Publikováno v: The Journal of Biological Chemistry

Background: The function of CsoR/RcnR-like protein InrS was unknown. Results: InrS and CsoR metal complexes have similar spectra, but InrS senses nickel not copper. Conclusion: InrS detects cytosolic nickel with tighter KD than the other metal sensor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::251aa6d66271ab024d941d7d5c89b1e1
http://europepmc.org/articles/PMC3320959

Trans-oligomerization of duplicated aminoacyl-tRNA synthetases maintains genetic code fidelity under stress

Autor: Javier Santamaría-Gómez, M.A. Rubio, Carl J. Patterson, Mauro Napolitano, Jesús A. G. Ochoa de Alda, Ignacio Luque, Nigel J. Robinson, Andrew W. Foster, Roque Bru-Martínez

Publikováno v: RUA. Repositorio Institucional de la Universidad de Alicante
Universidad de Alicante (UA)
Nucleic acids research, 2015, Vol.43(20), pp.9905-9917 [Peer Reviewed Journal]
idUS. Depósito de Investigación de la Universidad de Sevilla
instname
Nucleic Acids Research
Digital.CSIC. Repositorio Institucional del CSIC

Aminoacyl-tRNA synthetases (aaRSs) play a key role in deciphering the genetic message by producing charged tRNAs and are equipped with proofreading mechanisms to ensure correct pairing of tRNAs with their cognate amino acid. Duplicated aaRSs are very

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78237ed09051f12e68670c6056f5d717
http://hdl.handle.net/10045/51656

Cyanobacterial metallochaperone inhibits deleterious side reactions of copper

Autor: Nigel J. Robinson, Andrew W. Foster, Ivano Bertini, Steve Tottey, Isabella C. Felli, Rafael Pernil, Samantha J. Dainty, Carl J. Patterson, Anna Pavelkova, Kevin J. Waldron, Lucia Banci

Publikováno v: Proceedings of the National Academy of Sciences
ICT FP7 Publications Database
Europe PubMed Central

Copper metallochaperones supply copper to cupro-proteins through copper-mediated protein-protein-interactions and it has been hypothesized that metallochaperones thereby inhibit copper from causing damage en route . Evidence is presented in support o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90ce97ed648308b69cd0a6cca7685a97

Co(ii)-detection does not follow Kco(ii) gradient: channelling in Co(ii)-sensing

Autor: Nigel J. Robinson, Samantha J. Dainty, Victoria A. Money, Carl J. Patterson, Rafael Pernil, Clare E. Henry, Andrew W. Foster, Buddhapriya Chakrabarti

Publikováno v: Metallomics, 2013, Vol.5(4), pp.352-362 [Peer Reviewed Journal]

The MerR-like transcriptional activator CoaR detects surplus Co(ll) to regulate Co(ll) efflux in a cyanobacterium. This organism also has cytosolic metal-sensors from three further families represented by Zn(ll)-sensors ZiaR and Zur plus Ni(ll)-senso

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01551c90529d76f1d770b6124f93a31b
https://doi.org/10.1039/c3mt20241k