Harmless or Threatening? Interpreting the Results of Molecular Diagnosis in the Context of Virus-Host Relationships

Autor: Fábio A. Abade dos Santos, Sara J. Portela, Teresa Nogueira, Carina L. Carvalho, Rita de Sousa, Margarida D. Duarte

Publikováno v: Frontiers in Microbiology, Vol 12 (2021)

Molecular methods, established in the 1980s, expanded and delivered tools for the detection of vestigial quantities of nucleic acids in biological samples. Nucleotide sequencing of these molecules reveals the identity of the organism it belongs to. H

Externí odkaz: https://doaj.org/article/30e69e2416cc4130a8250129c64e7d60

A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen® or Taqman® Technologies

Autor: Fábio A. Abade dos Santos, Carina L. Carvalho, Maria C. Peleteiro, Francisco Parra, Margarida D. Duarte

Publikováno v: Viruses, Vol 13, Iss 4, p 715 (2021)

In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Th

Externí odkaz: https://doaj.org/article/91ee0ca0c4af448d9751077df3d0df39

Detection of Recombinant Hare Myxoma Virus in Wild Rabbits (Oryctolagus cuniculus algirus)

Autor: Fábio A. Abade dos Santos, Carina L. Carvalho, Andreia Pinto, Ranjit Rai, Madalena Monteiro, Paulo Carvalho, Paula Mendonça, Maria C. Peleteiro, Francisco Parra, Margarida D. Duarte

Publikováno v: Viruses, Vol 12, Iss 10, p 1127 (2020)

In late 2018, an epidemic myxomatosis outbreak emerged on the Iberian Peninsula leading to high mortality in Iberian hare populations. A recombinant Myxoma virus (strains MYXV-Tol and ha-MYXV) was rapidly identified, harbouring a 2.8 kbp insertion co

Externí odkaz: https://doaj.org/article/f911bcc9fe524009a6db7440420984a8

Recombinant myxoma virus infection associated with high mortality in rabbit farming (Oryctolaguscuniculus)

Autor: Paula Mendonça, Paulo de Tarso Camillo de Carvalho, Carina L. Carvalho, Madalena Monteiro, Fábio A Abade Dos Santos, Maria C. Peleteiro, Margarida Duarte

Publikováno v: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

Myxomatosis is an emergent disease in the Iberian hare, having been considered a rabbit disease for decades. Genome sequencing of the strains obtained from Iberian hares with myxomatosis showed these to be distinct from the classical ones that circul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c874b76f4b90ff556a799d1185537269
https://doi.org/10.1111/tbed.13899

Simple method for establishing primary Leporidae skin fibroblast cultures

Autor: Teresa Fagulha, Margarida Duarte, Margarida Henriques, Isabel Almeida, Fernanda Rammos, Sílvia C. Barros, Tiago Luís, Fábio A Abade Dos Santos, Carina L. Carvalho

Publikováno v: Cells
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Cells, Vol 10, Iss 2100, p 2100 (2021)

Research Areas: Cell Biology Commercial hare and rabbit immortalized cell lines are extremely limited regarding the many species within the lagomorpha order. To overcome this limitation, researchers and technicians must establish primary cell culture

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31f76fc931823b7ef7bd70c4c209c94b
https://hdl.handle.net/10400.5/22455