Výsledky vyhledávání - "Caride, Ariel"

Akademický článek

Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments.

Autor: Caride, Ariel¹ (AUTHOR), Jang, Jin Sung² (AUTHOR), Shi, Geng-Xian¹ (AUTHOR), Lenz, Sam¹ (AUTHOR), Zhong, Jian¹ (AUTHOR), Kim, Kwan Hyun¹ (AUTHOR), Allen, Mariet³ (AUTHOR), Robertson, Keith D.⁴ (AUTHOR), Farrugia, Gianrico⁵ (AUTHOR), Ordog, Tamas^1,5,6,7 (AUTHOR), Ertekin-Taner, Nilüfer^3,8 (AUTHOR), Lee, Jeong-Heon^1,9,10 (AUTHOR) Lee.JeongHeon@mayo.edu

Publikováno v: BMC Genomics. 4/4/2023, Vol. 24 Issue 1, p1-13. 13p.

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Additional file 2 of Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Autor: Caride, Ariel, Jang, Jin Sung, Shi, Geng-Xian, Lenz, Sam, Zhong, Jian, Kim, Kwan Hyun, Allen, Mariet, Robertson, Keith D., Farrugia, Gianrico, Ordog, Tamas, Ertekin-Taner, Nilüfer, Lee, Jeong-Heon

Additional file 2: Figure S1. DNAchrom shows a linear correlation with the amounts of chromatin input measured by purified DNA in individual sample type. DNA amount was directly measured in chromatin input by the Qubit dsDNA high sensitivity assay an

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Additional file 4 of Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Additional file 4: Figure S3. ChIP yield negatively correlates with the enrichment of positive targets. A: The profiles of DNA size purified from chromatin input and ChIPed chromatin. DNA was purified from input chromatin and ChIPed chromatin-antibod

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Additional file 11 of Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Additional file 11: Figure S7. The analysis after sorting shows the consistency of experimental outcome. A-D: Collected data was sorted following tissue weight (mg) (A), chromatin input by purified DNA (µg) (B), ChIP yield (%) (C), and peak number (

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Additional file 5 of Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Additional file 5: Figure S4. Titration-based normalization of antibody amount to the optimal titer leads to consistent ChIP yield and improved data quality. A-D: Fixed 0.25 µg of anti-H3K27ac antibody or normalized amounts to the optimal titer were

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Additional file 7 of Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Additional file 7: Figure S5. The quality and consistency of ChIP-seq libraries generated by the titration-based normalization approach. A-B: Correlation analysis between ChIP-seq datasets generated from different antibody titers. The Pearson’s cor

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Additional file 6 of Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Additional file 6: Table S2. Mapping results of ChIP-seq libraries used in Fig. 3. Raw sequencing reads were processed and analyzed using the ChIP-seq analysis package in the Partek Flow software. BWA aligner was used for mapping to the human referen

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Additional file 8 of Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Additional file 8: Figure S6. The profiles of purified DNA from chromatin input. DNA was purified from input chromatin after cross-linking reversal, RNase treatment, and proteinase K treatment. Purified DNA was analyzed by the Fragment analyzer (FA).

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Akademický článek

Alternative Pathways for Association and Dissociation of the Calmodulin-binding Domain of Plasma Membrane Ca2+-ATPase Isoform 4b (PMCA4b)

Autor: Penniston, John T., Caride, Ariel J., Strehler, Emanuel E.

Publikováno v: In Journal of Biological Chemistry 24 August 2012 287(35):29664-29671

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