Sensing with Nanopores and Aptamers: A Way Forward

Autor: Lucile Reynaud, Aurélie Bouchet-Spinelli, Camille Raillon, Arnaud Buhot

Publikováno v: Sensors, Vol 20, Iss 16, p 4495 (2020)

In the 90s, the development of a novel single molecule technique based on nanopore sensing emerged. Preliminary improvements were based on the molecular or biological engineering of protein nanopores along with the use of nanotechnologies developed i

Externí odkaz: https://doaj.org/article/d618d20371f845eeac2cf36654d8cc61

Discrimination of α-Thrombin and γ-Thrombin Using Aptamer-Functionalized Nanopore Sensing

Autor: Jean-Marc Janot, Arnaud Buhot, Aurélie Bouchet-Spinelli, Sebastien Balme, Lucile Reynaud, Camille Raillon

Publikováno v: Analytical Chemistry
Analytical Chemistry, 2021, 93 (22), pp.7889-7897. ⟨10.1021/acs.analchem.1c00461⟩

International audience; Protein detection and identification at the single-molecule level are major challenges in many biotechnological fields. Solid-state nanopores have raised attention as label-free biosensors with high sensitivity. Here, we use s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25e57465fee45fcb2e5dc099bcaac942
https://doi.org/10.1021/acs.analchem.1c00461

Biosensing extracellular vesicles: contribution of biomolecules in affinity-based methods for detection and isolation

Autor: Camille Raillon, Yoann Roupioz, M. Gaillard, Vincent Agache, Guillaume Nonglaton, Aurélie Thuaire

Publikováno v: Analyst
Analyst, Royal Society of Chemistry, 2020, 145 (6), pp.1997-2013. ⟨10.1039/c9an01949a⟩
Analyst, 2020, 145 (6), pp.1997-2013. ⟨10.1039/c9an01949a⟩
Analyst, Royal Society of Chemistry, 2020, 145 (6), pp.1997-2013. ⟨10.1039/C9AN01949A⟩

Extracellular Vesicles (EVs) are lipid vesicles secreted by cells that allow intercellular communication. They are decorated with surface proteins, which are membrane proteins that can be targeted by biochemical techniques to isolate EVs from backgro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fec1bd5309bdb9886ef3d1f31915f9b
https://hal.archives-ouvertes.fr/hal-02946413

Opto-electronic nose - temperature and VOC concentration effects on the equilibrium response

Autor: Raphael Mathey, Charlotte Hurot, Yanxia Hou, Jonathan S. Weerakkody, Sophie Brenet, Camille Raillon, Arnaud Buhot, Thierry Livache

Publikováno v: 2019 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN)
2019 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), May 2019, Fukuoka, Japan. pp.1-3, ⟨10.1109/ISOEN.2019.8823292⟩

Electronic Nose (eN) systems use sensor arrays containing multiple probes to generate fingerprints for the sensitive and selective detection of Volatile Organic Compounds (VOCs). In this paper, we analyzed the effect of VOC concentrations and of temp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9210920c312638970066f2a5e9ef3cd2
https://hal.archives-ouvertes.fr/hal-02376347

Nanopore Detection of Single Molecule RNAP–DNA Transcription Complex

Autor: Pascal Cousin, Floriano Traversi, E. Garcia-Cordero, Nouria Hernandez, Aleksandra Radenovic, Camille Raillon

Publikováno v: Nano letters
Nano Letters

In the past decade, a number of single-molecule methods have been developed with the aim of investigating single protein and nucleic acid interactions. For the first time we use solid-state nanopore sensing to detect a single E. coli RNAP-DNA transcr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f409bb8c8879075c8fc662692443521e
https://doi.org/10.1021/nl3002827