Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Camille Bonte"'
Autor:
Toon Swings, David C. Marciano, Benu Atri, Rachel E. Bosserman, Chen Wang, Marlies Leysen, Camille Bonte, Thomas Schalck, Ian Furey, Bram Van den Bergh, Natalie Verstraeten, Peter J. Christie, Christophe Herman, Olivier Lichtarge, Jan Michiels
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.
Externí odkaz:
https://doaj.org/article/07d39f442b264873bcd3c1d920a2d3d7
Autor:
Toon Swings, Bram Weytjens, Natalie Verstraeten, Jan Michiels, Kathleen Marchal, Thomas Schalck, Camille Bonte
Publikováno v:
Molecular Biology and Evolution
MOLECULAR BIOLOGY AND EVOLUTION
MOLECULAR BIOLOGY AND EVOLUTION
Efficient production of ethanol for use as a renewable fuel requires organisms with a high level of ethanol tolerance. However, this trait is complex and increased tolerance therefore requires mutations in multiple genes and pathways. Here, we use ex
Autor:
Rachel E. Bosserman, Ian M. Furey, Toon Swings, Benu Atri, Peter J. Christie, Olivier Lichtarge, Christophe Herman, Chen Wang, Thomas Schalck, Camille Bonte, David C. Marciano, Natalie Verstraeten, Marlies Leysen, Bram Van den Bergh, Jan Michiels
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Nature Communications
Nature Communications
CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flipp