Unveiling the role of PUS7-mediated pseudouridylation in host protein interactions specific for the SARS-CoV-2 RNA genome

Autor: Roberto Giambruno, Elsa Zacco, Camilla Ugolini, Andrea Vandelli, Logan Mulroney, Manfredi D’Onghia, Bianca Giuliani, Elena Criscuolo, Matteo Castelli, Nicola Clementi, Massimo Clementi, Nicasio Mancini, Tiziana Bonaldi, Stefano Gustincich, Tommaso Leonardi, Gian Gaetano Tartaglia, Francesco Nicassio

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 34, Iss , Pp 102052- (2023)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a positive single-stranded RNA virus, engages in complex interactions with host cell proteins throughout its life cycle. While these interactions enable the host to recognize and inhibit v

Externí odkaz: https://doaj.org/article/6eb7df49e017486c8f0fe36a0099dc99

Nanopore ReCappable sequencing maps SARS-CoV-2 5' capping sites and provides new insights into the structure of sgRNAs

Autor: Camilla Ugolini, Logan Mulroney, Adrien Leger, Matteo Castelli, Elena Criscuolo, Maia Kavanagh Williamson, Andrew D Davidson, Abdulaziz Almuqrin, Roberto Giambruno, Miten Jain, Gianmaria Frigè, Hugh Olsen, George Tzertzinis, Ira Schildkraut, Madalee G Wulf, Ivan R Corrêa, Laurence Ettwiller, Nicola Clementi, Massimo Clementi, Nicasio Mancini, Ewan Birney, Mark Akeson, Francesco Nicassio, David A Matthews, Tommaso Leonardi

Publikováno v: Ugolini, C, Mulroney, L, Leger, A, Castelli, M, Criscuolo, E, Kavanagh Williamson, M, Davidson, A D, Almuqrin, A M, Giambruno, R, Jain, M, Frige, G, Olsen, H, Tzertzinis, G, Schildkraut, I, Wulf, M G, Corrêa Jr, I R, Ettwiller, L, Clementi, N, Clementi, M, Mancini, N, Birney, E, Akeson, M, Nicassio, F, Matthews, D A & Leonardi, T 2022, ' Nanopore ReCappable sequencing maps SARS-CoV-2 5′ capping sites and provides new insights into the structure of sgRNAs ', Nucleic Acids Research, vol. 50, no. 6, gkac144, pp. 3475-3489 . https://doi.org/10.1093/nar/gkac144

The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA sequencing can recover full-length tran

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::439c61090bb65d43ceadd3bc367925e4
https://pubmed.ncbi.nlm.nih.gov/35244721