Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Calvin B.L. James"'
Publikováno v:
BioTechniques, Vol 60, Iss 6, Pp 293-298 (2016)
RNA isolation from pancreatic islets poses unique challenges. Here, we present a reproducible means of obtaining high-quality RNA from juvenile rodent islets in sufficient quantities for use in ex vivo expression studies. Tissue was extracted from fe
Externí odkaz:
https://doaj.org/article/f3afcc2d365d4a7d8d1fae7ba71971b1
Publikováno v:
BioTechniques, Vol 22, Iss 6, Pp 1024-1026 (1997)
Externí odkaz:
https://doaj.org/article/e671abba8905458aab6cdebb74f8589d
Publikováno v:
Experimental Physiology. 98:1301-1311
Diminished or inappropriate secretion of insulin is associated with type II diabetes. The cellular/molecular mechanism coupled with the regulation of insulin secretion is still under intense investigation. Divalent ion zinc (Zn(2+)) is co-packaged an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::61a93122c33d5015ff709c3a4a525850
https://doi.org/10.1113/expphysiol.2013.072348
https://doi.org/10.1113/expphysiol.2013.072348
Publikováno v:
BioTechniques. 60(6)
RNA isolation from pancreatic islets poses unique challenges. Here, we present a reproducible means of obtaining high-quality RNA from juvenile rodent islets in sufficient quantities for use in ex vivo expression studies. Tissue was extracted from fe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8a8a024febbe298f1a442dc798dac8d
https://pubmed.ncbi.nlm.nih.gov/27286806
https://pubmed.ncbi.nlm.nih.gov/27286806
Publikováno v:
Biochemical and biophysical research communications. 157(2)
Summary Incorporation of 32 P from γ-labeled ATP into a number of polypeptides in HeLa whole cell and nuclear extracts was dependent on added double-stranded DNA or poly(dI-dC), but not denatured or supercoiled DNA. DNA-dependent phosphorylation of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4182d3520de108486720b79086ed7a31
https://pubmed.ncbi.nlm.nih.gov/3202865
https://pubmed.ncbi.nlm.nih.gov/3202865