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Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology

Autor: Casper Berger, Navya Premaraj, Raimond B. G. Ravelli, Kèvin Knoops, Carmen López-Iglesias, Peter J. Peters

Publikováno v: Nature Methods. 20:499-511

Cryogenic electron microscopy and data processing enable the determination of structures of isolated macromolecules to near-atomic resolution. However, these data do not provide structural information in the cellular environment where macromolecules

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb02057d37b8257ab82b775e995de3f0
https://doi.org/10.1038/s41592-023-01783-5

Correlated Cryo Super-Resolution Light and Cryo-Electron Microscopy on Mammalian Cells Expressing the Fluorescent Protein rsEGFP2

Autor: Maarten W. Tuijtel, Thomas H. Sharp, Abraham J. Koster, Frank G. A. Faas

Publikováno v: Small Methods. WILEY-V C H VERLAG GMBH
Small Methods

Super-resolution light microscopy (SRM) enables imaging of biomolecules within cells with nanometer precision. Cryo-fixation by vitrification offers optimal structure preservation of biological specimens and permits sequential cryo electron microscop

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b9c3360f5189592acf7f3582ff24a26
https://hdl.handle.net/1887/122762

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High-vacuum optical platform for cryo-CLEM (HOPE):A new solution for non-integrated multiscale correlative light and electron microscopy

Autor: Shuoguo Li, Shengliu Wang, Yang Shi, Xu Wei, Lasse Hyldgaard Klausen, Gang Ji, Mingdong Dong, Xiaojun Huang, Wei Ding, Fei Sun, Xiang Zhang, Tongxin Niu

Publikováno v: Li, S, Ji, G, Shi, Y, Klausen, L H, Niu, T, Wang, S, Huang, X, Ding, W, Zhang, X, Dong, M, Xu, W & Sun, F 2018, ' High-vacuum optical platform for cryo-CLEM (HOPE) : A new solution for non-integrated multiscale correlative light and electron microscopy ', Journal of Structural Biology, vol. 201, no. 1, pp. 63-75 . https://doi.org/10.1016/j.jsb.2017.11.002

Cryo-correlative light and electron microscopy (cryo-CLEM) offers a unique way to analyze the high-resolution structural information of cryo-vitrified specimen by cryo-electron microscopy (cryo-EM) with the guide of the search for unique events by cr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c63566342ba4e2c2758e31e800ff504
https://pure.au.dk/portal/da/publications/highvacuum-optical-platform-for-cryoclem-hope(b11db639-c279-4867-ba89-955cd14ff39c).html