A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast

Autor: Daniele Novarina, Andriana Koutsoumpa, Andreas Milias-Argeitis

Publikováno v: STAR protocols, 3(2):101358

CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b59223c189c9a3b19f7cc4fc3692af7
https://pubmed.ncbi.nlm.nih.gov/35712010

Protocellular CRISPR/Cas‐Based Diffusive Communication Using Transcriptional RNA Signaling

Autor: Shuo Yang, Alex Joesaar, Bas W. A. Bögels, Stephen Mann, Tom F. A. de Greef

Publikováno v: Angewandte Chemie. International Edition, 61, 26, pp. 1-9
Angewandte Chemie. International Edition, 61, 1-9
Angewandte Chemie-International Edition, 61(26):e202202436. Wiley
Angewandte Chemie (International Edition), 61(26)

Protocells containing enzyme-driven biomolecular circuits that can process and exchange information offer a promising approach for mimicking cellular features and developing molecular information platforms. Here, we employ synthetic transcriptional c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b340951cd355bd9216b49e3b3c6b4a3
https://doi.org/10.1002/anie.202202436

The Role of Patents and Licensing in the Governance of Human Genome Editing: A White Paper

Autor: Matthews, Duncan, Brown, Abbe, Gambini, Emanuela, McMahon, Aisling, Minssen, Timo, Nordberg, Ana, Sherkow, Jacob S., Wested, Jakob, Van Zimmeren, Esther

On 12 July 2021 the World Health Organization (WHO) Expert Advisory Committee on Developing Global Standards for Governance and Oversight of Human Genome Editing published a set of reports entitled Human Genome Editing: A Framework for Governance and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______1110::79b65d4fcaa5d0f26172611cd2700bed
https://lup.lub.lu.se/record/b8a16a1f-9313-47fb-8a8e-45d3e0ddff39

Development of β-globin gene correction in human hematopoietic stem cells as a potential durable treatment for sickle cell disease

Autor: Helen Segal, Sruthi Mantri, M. Kyle Cromer, Neehar Bhatia, Maria Grazia Roncarolo, Annalisa Lattanzi, Ciaran M. Lee, David DiGiusto, Rasmus O. Bak, Carsten T. Charlesworth, Josefin Kenrick, Jason Skowronski, Matthew H. Porteus, J. Fraser Wright, Richard L. Frock, Daniel P. Dever, Narae Talbott, Christopher A. Vakulskas, Joab Camarena, Gang Bao, Waracharee Srifa, Premanjali Lahiri, John F. Tisdale

Publikováno v: Sci Transl Med
Lattanzi, A, Camarena, J, Lahiri, P, Segal, H, Srifa, W, Vakulskas, C A, Frock, R L, Kenrick, J, Lee, C, Talbott, N, Skowronski, J, Cromer, M K, Charlesworth, C T, Bak, R O, Mantri, S, Bao, G, DiGiusto, D, Tisdale, J, Wright, J F, Bhatia, N, Roncarolo, M G, Dever, D P & Porteus, M H 2021, ' Development of β-globin gene correction in human hematopoietic stem cells as a potential durable treatment for sickle cell disease ', Science Translational Medicine, vol. 13, no. 598, eabf2444 . https://doi.org/10.1126/scitranslmed.abf2444

Sickle cell disease (SCD) is the most common monogenic serious disease with 300,000 births annually worldwide. SCD is autosomal recessive from a single point mutation in codon six of the β-globin gene (HBB) resulting in sickle hemoglobin. Ex vivo β

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::664c1c185e0b70b2cf6b0e1b79df95cb
https://doi.org/10.1126/scitranslmed.abf2444

CFAP45 deficiency causes situs abnormalities and asthenospermia by disrupting an axonemal adenine nucleotide homeostasis module

Autor: Hidetaka Shiratori, Claudius Werner, Kavita Praveen, Stef J.F. Letteboer, Petra Pennekamp, Petras P. Dzeja, Katrin Junger, Inga M. Höben, Franziska Rabert, Friederike Stumme, Julia Wallmeier, Isabella Aprea, Zeev Perles, Erica E. Davis, Judith A. Klinkenbusch, Israel Amirav, Yasin Memari, Yayoi Ikawa, Niki T. Loges, Diana Bracht, Wang Kyaw Twan, Kerstin Bartscherer, Tabea Nöthe-Menchen, Micha Aviram, Yuan Ping Pang, Rim Hjeij, Johanna Raidt, Heike Olbrich, Huda Mussaffi, Katsutoshi Mizuno, Nicola Horn, Timo Strünker, Heymut Omran, Sandra Cindric, Asaf Ta-Shma, Gerard W. Dougherty, Katsuyoshi Takaoka, Marius Ueffing, Thomas Kaiser, Katsura Minegishi, Jörg Große-Onnebrink, Hiroshi Hamada, Jana Gützlaff, Bernd Dworniczak, Luisa Biebach, Samuel M. Young, Orly Elpeleg, Nicholas Katsanis, Karsten Boldt, Takahiro Ide, Ronald Roepman

Publikováno v: Nature Communications, 11
Nature Communications, Vol 11, Iss 1, Pp 1-20 (2020)
Nature Communications
Nature Communications, 11, 1
Nature Communications, 11(1). Nature Publishing Group

Axonemal dynein ATPases direct ciliary and flagellar beating via adenosine triphosphate (ATP) hydrolysis. The modulatory effect of adenosine monophosphate (AMP) and adenosine diphosphate (ADP) on flagellar beating is not fully understood. Here, we de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d88a83337a6f0f647e8e4ba37a5d642
http://hdl.handle.net/2066/229376