Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system.

Autor: Guzmán-Herrador DL; Departamento de Biología Molecular, Universidad de Cantabria (UC), Santander 39011, Spain.; Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Universidad de Cantabria - Consejo Superior de Investigaciones Científicas - Sociedad para el Desarrollo de Cantabria (UC-CSIC-SODERCAN), Santander 39011, Spain., Fernández-Gómez A; Departamento de Biología Molecular, Universidad de Cantabria (UC), Santander 39011, Spain.; Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Universidad de Cantabria - Consejo Superior de Investigaciones Científicas - Sociedad para el Desarrollo de Cantabria (UC-CSIC-SODERCAN), Santander 39011, Spain., Depardieu F; Institut Pasteur, Université Paris Cité, Microbiology Department, Synthetic Biology, Paris 75015, France., Bikard D; Institut Pasteur, Université Paris Cité, Microbiology Department, Synthetic Biology, Paris 75015, France., Llosa M; Departamento de Biología Molecular, Universidad de Cantabria (UC), Santander 39011, Spain.; Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Universidad de Cantabria - Consejo Superior de Investigaciones Científicas - Sociedad para el Desarrollo de Cantabria (UC-CSIC-SODERCAN), Santander 39011, Spain.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Oct 22; Vol. 121 (43), pp. e2408509121. Date of Electronic Publication: 2024 Oct 16.

Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing.

Autor: Hu W; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia., Kumar A; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia.; Diagnostic Genomics, Monash Health Pathology, Monash Medical Centre, Clayton, Victoria, Australia., Ahmed SF; Department of Electrical and Electronic Engineering, The University of Melbourne, Parkville, Victoria, Australia.; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., Qi S; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia., Ma DKG; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia.; Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia., Chen H; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia., Singh GJ; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia., Casan JML; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia., Haber M; Children's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney, New South Wales, Australia.; School of Women's and Children's Health, UNSW Sydney, Sydney, New South Wales, Australia., Voskoboinik I; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia., McKay MR; Department of Electrical and Electronic Engineering, The University of Melbourne, Parkville, Victoria, Australia.; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., Trapani JA; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia., Ekert PG; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia.; Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.; Children's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney, New South Wales, Australia.; School of Women's and Children's Health, UNSW Sydney, Sydney, New South Wales, Australia., Fareh M; Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia. mohamed.fareh@petermac.org.; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia. mohamed.fareh@petermac.org.

Publikováno v: Nature structural & molecular biology [Nat Struct Mol Biol] 2024 Nov; Vol. 31 (11), pp. 1702-1716. Date of Electronic Publication: 2024 Jul 01.

A modified glycosylase base editor without predictable DNA off-target effects.

Autor: Lian M; Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.; Research Unit of Generation of Large Animal Disease Models, Guangzhou, China., Chen T; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Chen M; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Peng X; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Yang Y; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China., Luo X; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Chi Y; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Wang J; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Tang C; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Zhou X; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Zhang K; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China., Qin C; Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China., Lai L; Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.; Research Unit of Generation of Large Animal Disease Models, Guangzhou, China.; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China.; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China., Zhou J; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China., Zou Q; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China.

Publikováno v: FEBS letters [FEBS Lett] 2024 Oct; Vol. 598 (20), pp. 2557-2565. Date of Electronic Publication: 2024 Jun 30.

Heritable gene editing in tomato through viral delivery of isopentenyl transferase and single-guide RNAs to latent axillary meristematic cells.

Autor: Liu D; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Ellison EE; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Myers EA; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Donahue LI; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Xuan S; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Swanson R; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Qi S; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Prichard LE; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Starker CG; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108., Voytas DF; Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Sep 24; Vol. 121 (39), pp. e2406486121. Date of Electronic Publication: 2024 Sep 16.

Protocol for establishing inducible CRISPRd system for blocking transcription factor-binding sites in human pluripotent stem cells.

Autor: Matsui S; Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address: matsui.satoshi.3p8@osaka-u.ac.jp., Shiley JR; Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA., Buckley M; Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA., Lim HW; Department of Biomedical Informatics, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA., Hu YC; Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA., Mayhew CN; Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address: christopher.mayhew@cchmc.org., Iwafuchi M; Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address: makiko.iwafuchi@cchmc.org.

Publikováno v: STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103233. Date of Electronic Publication: 2024 Aug 11.