Zobrazeno 1 - 10
of 12 318
pro vyhledávání: '"CRISPR–Cas9"'
Autor:
Rahul Kumar, Sankar Prasad Das, Burhan Uddin Choudhury, Amit Kumar, Nitish Ranjan Prakash, Ramlakhan Verma, Mridul Chakraborti, Ayam Gangarani Devi, Bijoya Bhattacharjee, Rekha Das, Bapi Das, H. Lembisana Devi, Biswajit Das, Santoshi Rawat, Vinay Kumar Mishra
Publikováno v:
Biological Research, Vol 57, Iss 1, Pp 1-23 (2024)
Abstract Conventional pre-genomics breeding methodologies have significantly improved crop yields since the mid-twentieth century. Genomics provides breeders with advanced tools for whole-genome study, enabling a direct genotype–phenotype analysis.
Externí odkaz:
https://doaj.org/article/d62676a93f7042e494e09d8d8a92759f
Publikováno v:
The EuroBiotech Journal, Vol 8, Iss 4, Pp 213-229 (2024)
The precise prediction of off-target effects in CRISPR-Cas9 genome editing is critical for ensuring the safety and efficacy of this powerful tool. This study leverages machine learning techniques to predict off-target cleavage sites and investigate t
Externí odkaz:
https://doaj.org/article/e723cd04e3354f1f92a28023e9e5ba80
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-14 (2024)
Abstract Conditional regulation is a highly beneficial system for studying the function of essential genes in Plasmodium falciparum and dimerizable Cre recombinase (DiCre) is a recently adapted conditional regulation system suitable for this purpose.
Externí odkaz:
https://doaj.org/article/847f659377b945b3bc401aa5f7bcb01b
Publikováno v:
BMC Plant Biology, Vol 24, Iss 1, Pp 1-12 (2024)
Abstract Background Heterosis is a common phenomenon in plants and has been extensively applied in crop breeding. However, the superior traits in the hybrids can only be maintained in the first generation but segregate in the following generations. M
Externí odkaz:
https://doaj.org/article/61e6e9c24bee483dbb68264f22aa4aa6
Autor:
Javier Poyatos-García, Patricia Soblechero-Martín, Alessandro Liquori, Andrea López-Martínez, Pilar Maestre, Elisa González-Romero, Rafael P. Vázquez-Manrique, Nuria Muelas, Gema García-García, Jessica Ohana, Virginia Arechavala-Gomeza, Juan J. Vílchez
Publikováno v:
Skeletal Muscle, Vol 14, Iss 1, Pp 1-14 (2024)
Abstract Background Gene editing therapies in development for correcting out-of-frame DMD mutations in Duchenne muscular dystrophy aim to replicate benign spontaneous deletions. Deletion of 45–55 DMD exons (del45–55) was described in asymptomatic
Externí odkaz:
https://doaj.org/article/04805daa00ca464381398824273732b2
Publikováno v:
Сибирский онкологический журнал, Vol 23, Iss 4, Pp 152-161 (2024)
Purpose of the study: analysis of modern scientific data on the molecular mechanisms of the CRISPR-Cas9 system in gene editing, advantages and disadvantages in cancer research and the development of new treatment methods. Material and Methods. A comp
Externí odkaz:
https://doaj.org/article/681c2cc948994722aec234a6c8a53871
Autor:
Yu C. J. Chey, Mark A. Corbett, Jayshen Arudkumar, Sandra G. Piltz, Paul Q. Thomas, Fatwa Adikusuma
Publikováno v:
BMC Biology, Vol 22, Iss 1, Pp 1-15 (2024)
Abstract Background The development of sequence-specific precision treatments like CRISPR gene editing therapies for Duchenne muscular dystrophy (DMD) requires sequence humanized animal models to enable the direct clinical translation of tested strat
Externí odkaz:
https://doaj.org/article/dcea31a8dfee493680c161e9f4f7c5c9
Autor:
Xiao-Jun Yu, Yuan-Ting Zhao, Haimiti Abudouaini, Peng Zou, Tian-Qi Li, Xiao-Fan Bai, Shan-Xi Wang, Jian-Bin Guan, Meng-wei Li, Xiao-dong Wang, Ying-guang Wang, Ding-Jun Hao
Publikováno v:
Journal of Nanobiotechnology, Vol 22, Iss 1, Pp 1-29 (2024)
Abstract Methods Single-cell transcriptomics and high-throughput transcriptomics were used to screen factors significantly correlated with intervertebral disc degeneration (IDD). Expression changes of CFIm25 were determined via RT-qPCR and Western bl
Externí odkaz:
https://doaj.org/article/266619a5bda24d74aee1a02022df6fff
CRISPR-Cas9 gene editing strengthens cuproptosis/chemodynamic/ferroptosis synergistic cancer therapy
Autor:
Xiaoyu Wu, Zijun Bai, Hui Wang, Hanqing Wang, Dahai Hou, Yunzhu Xu, Guanqun Wo, Haibo Cheng, Dongdong Sun, Weiwei Tao
Publikováno v:
Acta Pharmaceutica Sinica B, Vol 14, Iss 9, Pp 4059-4072 (2024)
Copper-based nanomaterials demonstrate promising potential in cancer therapy. Cu+ efficiently triggers a Fenton-like reaction and further consumes the high level of glutathione, initiating chemical dynamic therapy (CDT) and ferroptosis. Cuproptosis,
Externí odkaz:
https://doaj.org/article/23d0e7e6465b483e80125ac9ed8ce80c
Publikováno v:
Biomarker Research, Vol 12, Iss 1, Pp 1-29 (2024)
Abstract Gene therapy utilizes nucleic acid drugs to treat diseases, encompassing gene supplementation, gene replacement, gene silencing, and gene editing. It represents a distinct therapeutic approach from traditional medications and introduces nove
Externí odkaz:
https://doaj.org/article/d83f835ce9d34ae3ad58e0924c1b7bb6