Zobrazeno 1 - 10
of 66
pro vyhledávání: '"CRISPR, clustered regularly interspaced short palindromic repeat"'
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
CRISPR–Cas technologies contribute to enhancing our understanding of plant gene functions, and to the precise breeding of crop traits. Here, we review the latest progress in plant genome editing, focusing on emerging CRISPR–Cas systems, DNA-free
Externí odkaz:
https://doaj.org/article/b9dcae64a1684f6c9be677b06edb5dec
Autor:
Yasuhiro Ito
Publikováno v:
Frontiers in Plant Science, Vol 15 (2024)
Generating CRISPR/Cas9-mediated mutants in tomato (Solanum lycopersicum L.) involves screening shoots regenerated from cultured cells transformed with a T-DNA harboring sequences encoding Cas9 and single guide RNAs (sgRNAs). Production of transforman
Externí odkaz:
https://doaj.org/article/a857a929426942afb833b3f7e5eebf0c
Publikováno v:
Frontiers in Plant Science, Vol 14 (2024)
Externí odkaz:
https://doaj.org/article/e38518d6cd99415baea2f826477f5fcf
Autor:
Yuxiang Wang, Athar Khalil, Amina Kamar, Mengyan Du, Trang Dinh, Christopher McFarland, Zhenghe Wang
Publikováno v:
Frontiers in Genetics, Vol 14 (2023)
Immune checkpoint inhibitors (ICIs) have revolutionized cancer immunotherapy by reinvigorating antitumor immune responses, but their efficacy remains limited in most patients. To address this challenge and optimize Immune check inhibitor treatment, u
Externí odkaz:
https://doaj.org/article/20c4cc8cf10e4da79882b7dc91730668
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Epigenetic dysregulation is an important determinant of many pathological conditions and diseases. Designer molecules that can specifically target endogenous DNA sequences provide a means to therapeutically modulate gene function. The prokaryote-deri
Externí odkaz:
https://doaj.org/article/89557838df4841e4825115c9d5a7344c
Autor:
Jose S. Campos, Sarah E. Henrickson
Publikováno v:
Frontiers in Immunology, Vol 13 (2022)
Inborn errors of immunity (IEIs) are a group of more than 450 monogenic disorders that impair immune development and function. A subset of IEIs blend increased susceptibility to infection, autoimmunity, and malignancy and are known collectively as pr
Externí odkaz:
https://doaj.org/article/4e9b7f8fe6d14f90907901223ed12348
Autor:
Benjamin C. Houghton, Neelam Panchal, Simone A. Haas, Kay O. Chmielewski, Markus Hildenbeutel, Thomas Whittaker, Claudio Mussolino, Toni Cathomen, Adrian J Thrasher, Claire Booth
Publikováno v:
Frontiers in Genome Editing, Vol 4 (2022)
X-linked lymphoproliferative disease is a rare inherited immune disorder, caused by mutations or deletions in the SH2D1A gene that encodes an intracellular adapter protein SAP (Slam-associated protein). SAP is essential for mediating several key immu
Externí odkaz:
https://doaj.org/article/e1802e6cee45487398ad67ff6d224f5a
Publikováno v:
Frontiers in Genome Editing, Vol 3 (2021)
CRISPR/Cas-based genome editing technologies have the potential to fast-track large-scale crop breeding programs. However, the rigid cell wall limits the delivery of CRISPR/Cas components into plant cells, decreasing genome editing efficiency. Establ
Externí odkaz:
https://doaj.org/article/0d48ef6ee36e44a4954b538c919b8639
Autor:
Keisuke Sekine
Publikováno v:
Frontiers in Genetics, Vol 12 (2021)
Recent progress in the field of organoid-based cell culture systems has enabled the use of patient-derived cells in conditions that resemble those in cancer tissue, which are better than two-dimensional (2D) cultured cell lines. In particular, organo
Externí odkaz:
https://doaj.org/article/7c56f7a611d3464ba590446a27eeaa59
Autor:
Kaitlin M. Dailey, Reed I. Jacobson, Paige R. Johnson, Taylor J. Woolery, Jiha Kim, Rick J. Jansen, Sanku Mallik, Amanda E. Brooks
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
The tumor microenvironment is characterized by anomalous vascularization, hypoxia, and acidity at the core of solid tumors that culminates in concentrated necrosis and immune system dysregulation among other effects. While this environment presents s
Externí odkaz:
https://doaj.org/article/ffd284c925e3450caf21982232cd4679