Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting

Autor: Oscar P. B. Wiklander, Joel Z. Nordin, Aisling O'Loughlin, Ylva Gustafsson, Giulia Corso, Imre Mäger, Pieter Vader, Yi Lee, Helena Sork, Yiqi Seow, Nina Heldring, Lydia Alvarez-Erviti, CI Edvard Smith, Katarina Le Blanc, Paolo Macchiarini, Philipp Jungebluth, Matthew J. A. Wood, Samir EL Andaloussi

Publikováno v: Journal of Extracellular Vesicles, Vol 4, Iss 0, Pp 1-8 (2015)

Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication in a diverse range of biological processes. For future therapeutic applications and for EV biology research in general, understanding the in vivo fate of

Externí odkaz: https://doaj.org/article/0d7374c1eade4f03b61583b05e54f879

Micro-minicircle Gene Therapy: Implications of Size on Fermentation, Complexation, Shearing Resistance, and Expression

Autor: Sofia Stenler, Oscar PB Wiklander, Maria Badal-Tejedor, Janne Turunen, Joel Z Nordin, David Hallengärd, Britta Wahren, Samir EL Andaloussi, Mark W Rutland, CI Edvard Smith, Karin E Lundin, Pontus Blomberg

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 3, Iss C (2014)

The minicircle (MC), composed of eukaryotic sequences only, is an interesting approach to increase the safety and efficiency of plasmid-based vectors for gene therapy. In this paper, we investigate micro-MC (miMC) vectors encoding small regulatory RN

Externí odkaz: https://doaj.org/article/f6a327b6051943f3936632a5f1af2970

Expression Analysis in Multiple Muscle Groups and Serum Reveals Complexity in the MicroRNA Transcriptome of the mdx Mouse with Implications for Therapy

Autor: Thomas C Roberts, K Emelie M Blomberg, Graham McClorey, Samir EL Andaloussi, Caroline Godfrey, Corinne Betts, Thibault Coursindel, Michael J Gait, CI Edvard Smith, Matthew JA Wood

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 1, Iss C (2012)

MicroRNAs (miRNAs) are a class of small RNAs that regulate gene expression and are implicated in wide-ranging cellular processes and pathological conditions including Duchenne muscular dystrophy (DMD). We have compared differential miRNA expression i

Externí odkaz: https://doaj.org/article/e9477d02be4e40489e38f8dbfc780860

Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency

Autor: Adil Doganay Duru, Giulia Corso, Ci Edvard Smith, Pieter Vader, Samir El Andaloussi, Raymond M. Schiffelers, Jeremy P Bost, Joel Z. Nordin, Mattias Hällbrink, Kärt Padari, Margus Pooga, Oscar P. B. Wiklander, Helena Sork, Eman M. Zaghloul, Helerin Margus, Matthew J.A. Wood, Burcu Bestas, Janne J. Turunen

Publikováno v: Molecular Therapy. Nucleic Acids
Molecular therapy. Nucleic acids, 5. Nature Publishing Group
Molecular Therapy: Nucleic Acids, Vol 5, Iss C (2016)

The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a64b7d8947856f660a6186c6a95401f
http://europepmc.org/articles/PMC5014464

Novel endosomolytic compounds enable highly potent delivery of antisense oligonucleotides.

Autor: Bost JP; Department of Laboratory Medicine, Karolinska Institutet, Huddinge, 14157, Sweden. jeremy.bost@ki.se., Ojansivu M; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, 17177, Sweden., Munson MJ; Advanced Drug Delivery, Pharmaceutical Sciences, Biopharmaceutical R&D, AstraZeneca, Mölndal, 43150, Sweden., Wesén E; Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden., Gallud A; Advanced Drug Delivery, Pharmaceutical Sciences, Biopharmaceutical R&D, AstraZeneca, Mölndal, 43150, Sweden.; Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden., Gupta D; Department of Laboratory Medicine, Karolinska Institutet, Huddinge, 14157, Sweden., Gustafsson O; Department of Laboratory Medicine, Karolinska Institutet, Huddinge, 14157, Sweden., Saher O; Department of Laboratory Medicine, Karolinska Institutet, Huddinge, 14157, Sweden.; Department of Pharmaceutics and Industrial Pharmacy, Cairo University, Cairo, 11562, Egypt., Rädler J; Department of Laboratory Medicine, Karolinska Institutet, Huddinge, 14157, Sweden., Higgins SG; Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, United Kingdom., Lehto T; Department of Laboratory Medicine, Karolinska Institutet, Huddinge, 14157, Sweden.; Institute of Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia., Holme MN; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, 17177, Sweden., Dahlén A; Discovery Sciences, R&D, AstraZeneca, Gothenburg, 43150, Sweden., Engkvist O; Discovery Sciences, R&D, AstraZeneca, Gothenburg, 43150, Sweden., Strömstedt PE; Discovery Sciences, R&D, AstraZeneca, Gothenburg, 43150, Sweden., Andersson S; Discovery Sciences, R&D, AstraZeneca, Gothenburg, 43150, Sweden., Edvard Smith CI; Department of Laboratory Medicine, Karolinska Institutet, Huddinge, 14157, Sweden., Stevens MM; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, 17177, Sweden.; Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, United Kingdom., Esbjörner EK; Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden., Collén A; Advanced Drug Delivery, Pharmaceutical Sciences, Biopharmaceutical R&D, AstraZeneca, Mölndal, 43150, Sweden.; Projects, Research and Early Development, Cardiovascular, Renal and Metabolism, Biopharmaceuticals R&D, AstraZeneca, Gothenburg, 43150, Sweden., El Andaloussi S; Department of Laboratory Medicine, Karolinska Institutet, Huddinge, 14157, Sweden. samir.el-andaloussi@ki.se.

Publikováno v: Communications biology [Commun Biol] 2022 Mar 01; Vol. 5 (1), pp. 185. Date of Electronic Publication: 2022 Mar 01.