CCA-addition in the cold: Structural characterization of the psychrophilic CCA-adding enzyme from the permafrost bacterium Planococcus halocryophilus

Autor: Raphaël de Wijn, Kévin Rollet, Felix G.M. Ernst, Karolin Wellner, Heike Betat, Mario Mörl, Claude Sauter

Publikováno v: Computational and Structural Biotechnology Journal, Vol 19, Iss , Pp 5845-5855 (2021)

CCA-adding enzymes are highly specific RNA polymerases that add and maintain the sequence C-C-A at tRNA 3‘-ends. Recently, we could reveal that cold adaptation of such a polymerase is not only achieved at the expense of enzyme stability, but also a

Externí odkaz: https://doaj.org/article/483aeb0567bc4cec89987f2a9e1502ba

Adaptation of the Romanomermis culicivorax CCA-Adding Enzyme to Miniaturized Armless tRNA Substrates

Autor: Hennig, Oliver, Philipp, Susanne, Bonin, Sonja, Rollet, Kévin, Kolberg, Tim, Jühling, Tina, Betat, Heike, Sauter, Claude, Mörl, Mario

The mitochondrial genome of the nematode Romanomermis culicivorax encodes for miniaturized hairpin-like tRNA molecules that lack D- as well as T-arms, strongly deviating from the consensus cloverleaf. The single tRNA nucleotidyltransferase of this or

Externí odkaz: https://ul.qucosa.de/id/qucosa%3A88931
https://ul.qucosa.de/api/qucosa%3A88931/attachment/ATT-0/

Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme

Autor: Franz, Paul, Betat, Heike, Mörl, Mario

Publikováno v: BMC Microbiology (2016) 16:47 doi: 10.1186/s12866-016-0670-2

Background: To allow an immediate treatment of an infection with suitable antibiotics and bactericides or fungicides, there is an urgent need for fast and precise identification of the causative human pathogens. Methods based on DNA sequence comparis

Externí odkaz: https://ul.qucosa.de/id/qucosa%3A14762
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Unusual tRNA nucleotidyltransferases: Adaptation of the Romanomermis culicivorax CCA-adding enzyme towards armless tRNAs and characterization of the phosphodiesterase domain of the Escherichia coli CCA-adding enzyme

Autor: Philipp, Susanne

tRNAs spielen eine zentrale Rolle in jedem bekannten Organismus. Sie agieren als Adaptoren, um den Code der Nukleinsäuren in eine funktionale Aminosäuresequenz zu übersetzen. Ihre Struktur folgt einer hoch konservierten Kleeblattform, die aus dem

Externí odkaz: https://ul.qucosa.de/id/qucosa%3A78784
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CCA-addition in the cold: Structural characterization of the psychrophilic CCA-adding enzyme from the permafrost bacterium Planococcus halocryophilus

Autor: de Wijn, Raphaël, Rollet, Kévin, Ernst, Felix G.M., Wellner, Karolin, Betat, Heike, Mörl, Mario, Sauter, Claude

Publikováno v: Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal, Elsevier, 2021, 19, pp.5845-5855. ⟨10.1016/j.csbj.2021.10.018⟩
Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 5845-5855 (2021)

International audience; CCA-adding enzymes are highly specific RNA polymerases that add and maintain the sequence CC A at tRNA 3'-ends. Recently, we could reveal that cold adaptation of such a polymerase is not only achieved at the expense of enzyme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::2dd8584e0bc41fdba83bf3a2909b1f06
https://hal.archives-ouvertes.fr/hal-03419675/document