Zobrazeno 1 - 10
of 17 706
pro vyhledávání: '"CAS9"'
Autor:
Sebastian Brandner
Publikováno v:
Molecular Oncology, Vol 18, Iss 12, Pp 2842-2870 (2024)
Modelling of human diseases is an essential component of biomedical research, to understand their pathogenesis and ultimately, develop therapeutic approaches. Here, we will describe models of tumours of the central nervous system, with focus on intri
Externí odkaz:
https://doaj.org/article/e4ef6656aca54fac913d2b7f6b9d6e8f
Autor:
Takayuki Sakurai, Norio Takei, Yangxuan Wei, Marina Hayashi, Akiko Kamiyoshi, Hisaka Kawate, Satoshi Watanabe, Masahiro Sato, Takayuki Shindo
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-14 (2024)
Abstract Creating genetically modified (GM) animals using CRISPR/Cas mediated through the electroporation of two-cell stage embryos, rather than fertilized eggs, holds considerable potential. The full potential of genome editing using two-cell stage
Externí odkaz:
https://doaj.org/article/d7540f30240f4939a4f087793dc06a11
Autor:
Leilei Wu, Shutan Jiang, Meisong Shi, Tanglong Yuan, Yaqin Li, Pinzheng Huang, Yingqi Li, Erwei Zuo, Changyang Zhou, Yidi Sun
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-22 (2024)
Abstract Background The safety of CRISPR-based gene editing methods is of the utmost priority in clinical applications. Previous studies have reported that Cas9 cleavage induced frequent aneuploidy in primary human T cells, but whether cleavage-media
Externí odkaz:
https://doaj.org/article/a173f9a0e0c44725899cd86c3aaab2fd
Publikováno v:
Jichu yixue yu linchuang, Vol 44, Iss 10, Pp 1419-1427 (2024)
Objective To establish human and mouse pancreatic cancer cell strains stably expressing Cas9 protein, green fluorescent protein, red fluorescent proteins, luciferase-tdTomato, and to validate the activity of luciferase and gene editing of Cas9 functi
Externí odkaz:
https://doaj.org/article/9c04e5212a8949e5a2524655b50921ad
Autor:
Hillary Elrick, Kevin A. Peterson, Brandon J. Willis, Denise G. Lanza, Elif F. Acar, Edward J. Ryder, Lydia Teboul, Petr Kasparek, Marie-Christine Birling, David J. Adams, Allan Bradley, Robert E. Braun, Steve D. Brown, Adam Caulder, Gemma F. Codner, Francesco J. DeMayo, Mary E. Dickinson, Brendan Doe, Graham Duddy, Marina Gertsenstein, Leslie O. Goodwin, Yann Hérault, Lauri G. Lintott, K. C. Kent Lloyd, Isabel Lorenzo, Matthew Mackenzie, Ann-Marie Mallon, Colin McKerlie, Helen Parkinson, Ramiro Ramirez-Solis, John R. Seavitt, Radislav Sedlacek, William C. Skarnes, Damien Smedley, Sara Wells, Jacqueline K. White, Joshua A. Wood, International Mouse Phenotyping Consortium, Stephen A. Murray, Jason D. Heaney, Lauryl M. J. Nutter
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-15 (2024)
Abstract The International Mouse Phenotyping Consortium (IMPC) systematically produces and phenotypes mouse lines with presumptive null mutations to provide insight into gene function. The IMPC now uses the programmable RNA-guided nuclease Cas9 for i
Externí odkaz:
https://doaj.org/article/0788cc9d0e59472c90243baecc221f96
Publikováno v:
Journal of Advanced Research, Vol 63, Iss , Pp 73-90 (2024)
Introduction: Our previous work reveals a critical role of activation of neuronal Alox5 in exacerbating brain injury post seizures. However, whether neuronal Alox5 impacts the pathological process of epilepsy remains unknown. Objectives: To prove the
Externí odkaz:
https://doaj.org/article/2c7cc60d53ed4ac5b64bd5ce967d8684
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-23 (2024)
Abstract Since the introduction of next generation sequencing technologies, the field of epigenomics has evolved rapidly. However, most commonly used assays are enrichment-based methods and thus only semi-quantitative. Nucleosome occupancy and methyl
Externí odkaz:
https://doaj.org/article/d24fbde6bfc84185b1006c146eb010a9
Publikováno v:
BMC Research Notes, Vol 17, Iss 1, Pp 1-6 (2024)
Abstract Objective Insertion and deletion (indel) analysis of CRISPR-Cas guide RNAs (gRNAs) is crucial in gene editing to assess gRNA efficiency and indel frequency. This study evaluates the utility of CRISPResso2 with Oxford Nanopore sequencing data
Externí odkaz:
https://doaj.org/article/be93d1b323764efb99057021330392f8
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-11 (2024)
Abstract The contraction of CAG/CTG repeats is an attractive approach to correct the mutation that causes at least 15 neuromuscular and neurodegenerative diseases, including Huntington’s disease and Myotonic Dystrophy type 1. Contractions can be ac
Externí odkaz:
https://doaj.org/article/18056ebe522b4678a7b61f4ef8107959
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-9 (2024)
Abstract Due to limitations in conventional disease vector control strategies including the rise of insecticide resistance in natural populations of mosquitoes, genetic control strategies using CRISPR gene drive systems have been under serious consid
Externí odkaz:
https://doaj.org/article/83d9ac40bb234251b5065e133f5694e3