Zobrazeno 1 - 10
of 110
pro vyhledávání: '"C.J. Cole"'
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Publikováno v:
Gene. 162:29-35
Site-directed mutagenesis of the penDE gene and expression in Escherichia coli has produced recombinant acylcoenzyme A:isopenicillin N acyltransferase (re-AT) containing amino-acid substitutions in the proenzyme cleavage site (↓) region (Asp-Gly 10
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bea79d5d4d76cb0461fde8489a0f8713
https://doi.org/10.1016/0378-1119(95)00369-h
https://doi.org/10.1016/0378-1119(95)00369-h
Autor:
John D. Sutherland, Nicholas Morgan, Jack E. Baldwin, Robert M. Adlington, Inês A. C. Pereira, Nicholas P. Crouch, Inger Andersson, Steven C.J. Cole
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 4:1595-1600
The soluble, high-level expression in Escherichia coli of the deacetoxycephalosporin C synthase gene from Streptomyces clavuligerus and the substrate specificity of the recombinant enzyme are described.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6301ab35ce6e056c5bb3e10f4ba8d782
https://doi.org/10.1016/s0960-894x(01)80573-4
https://doi.org/10.1016/s0960-894x(01)80573-4
Publikováno v:
FEMS Microbiology Letters. 113:189-195
Trypsin-like enzymes from the entomopathogenic fungus Metarhizium anisopliae have been characterised. Two proteases with tryptic activity were purified by narrow range isoelectric focussing and affinity chromatography. One of these proteases, with an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91343c5a74db570272ef24a827836782
https://doi.org/10.1111/j.1574-6968.1993.tb06512.x
https://doi.org/10.1111/j.1574-6968.1993.tb06512.x
Autor:
John D. Sutherland, Jack E. Baldwin, Tobin Matthew Barry, Paul L. Skatrud, James R. Miller, Stephen C.J. Cole
Publikováno v:
Gene. 132:199-206
Subunit interaction in the formation of active acyl-coenzyme A:isopenicillin N acyltransferase (AT) has been investigated. Various AT derivatives were produced from altered Penicillium chrysogenum penDE genes placed in Escherichia coli expression sys
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e108ef534b4f8437720d52c4014ed96
https://doi.org/10.1016/0378-1119(93)90196-a
https://doi.org/10.1016/0378-1119(93)90196-a
Autor:
S.A. Josselyn, C.J. Cole
Publikováno v:
Learning and Memory: A Comprehensive Reference ISBN: 9780123705099
Previous studies have shown that long-term memory requires gene transcription, suggesting that regulatory mechanisms directing transcription may play a crucial role in memory formation. Here we examine the role of several related transcriptional regu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3a7f0ba10c5775bb333803e0f533daa7
https://doi.org/10.1016/b978-012370509-9.00030-9
https://doi.org/10.1016/b978-012370509-9.00030-9
Kniha
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Autor:
Giorgio Pelosi, Christopher J. Schofield, Stephen C.J. Cole, Pär Nordlund, Jack E. Baldwin, Janos Hajdu, Y. Fujishima
Publikováno v:
Journal of Molecular Biology. 242:712-714
Recombinant isopenicillin N synthase from Cephalosporium acremonium was expressed in Escherichia coli and the protein was purified. After nearly 5000 crystallization trials, the apo enzyme was crystallized by the hanging drop vapour diffusion techniq
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d2dce35edb7909096303e94478415db
https://doi.org/10.1006/jmbi.1994.1622
https://doi.org/10.1006/jmbi.1994.1622
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Autor:
James R. Miller, Jack E. Baldwin, Matthew B. Tobin, John D. Sutherland, Stephen C.J. Cole, Steven Kovacevic
Publikováno v:
FEMS microbiology letters. 121(1)
Using a high level Escherichia coli expression system for the Penicillium chrysogenum penDE gene, we have produced acyl-coenzyme A: isopenicillin N acyltransferase (AT) enzymes containing amino acid substitutions at three conserved Ser residues. Chos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc792d38261baade04bcf0585e88882b
https://pubmed.ncbi.nlm.nih.gov/8082826
https://pubmed.ncbi.nlm.nih.gov/8082826