Zobrazeno 1 - 10
of 239
pro vyhledávání: '"C.A.M.J.J. van den Hondel"'
Autor:
Muhammad Jahangir, Hye Kyong Kim, Young Hae Choi, Ibrahim B. Abdel-Farid, Robert Verpoorte, C.A.M.J.J. van den Hondel
Publikováno v:
Plant Science. 176:608-615
The metabolic interactions between three cultivars of Brassica rapa and three pathogenic fungi such as Leptosphaeria maculans , Aspergillus niger , and Fusarium oxysporum were investigated using 1 H nuclear magnetic resonance spectroscopy. Firstly, i
Publikováno v:
Fungal Genetics and Biology. 42:9-19
In this study, the efficiency of gene replacement in Aspergillus awamori between Agrobacterium-mediated transformation and CaCl(2)/PEG-mediated transformation was compared. For the genes, pyrG and gfaA, it was found that the homologous recombination
Publikováno v:
Applied Microbiology and Biotechnology 67 (2005) 1
Applied Microbiology and Biotechnology, 1, 67, 75-82
Applied Microbiology and Biotechnology, 67(1), 75-82
Applied Microbiology and Biotechnology, 1, 67, 75-82
Applied Microbiology and Biotechnology, 67(1), 75-82
Solid-state fermentation (SSF) with Aspergillus oryzae results in high levels of secreted protein. However, control mechanisms of gene expression in SSF have been only poorly studied. In this study we show that both glucoamylase (glaB) and protease (
Role of bacterial virulence proteins in Agrobacterium-mediated transformation of Aspergillus awamori
Publikováno v:
Fungal Genetics and Biology. 41:571-578
The Agrobacterium-mediated transformation of Aspergillus awamori was optimized using defined co-cultivation conditions, which resulted in a reproducible and efficient transformation system. Optimal co-cultivation conditions were used to study the rol
Autor:
Glyn Nelson, Marc R. Knight, A. J. Collis, J. G. M. Hessing, C.A.M.J.J. van den Hondel, Nick D. Read, J. R. S. Fincham, Peter J. Punt, A. Renwick, O. Kozlova-Zwinderman, C. P. Stanger
Publikováno v:
Molecular Microbiology. 52:1437-1450
Calcium signalling is little understood in filamentous fungi largely because easy and routine methods for calcium measurement in living hyphae have previously been unavailable. We have developed the recombinant aequorin method for this purpose. High
Autor:
David J. Jeenes, J.A Spencer, B.C Lokman, A Drint-Kuijvenhoven, Peter J. Punt, C.A.M.J.J. van den Hondel, D.A Archer
Publikováno v:
Journal of Biotechnology. 106:23-32
We have characterized growth and protein processing characteristics of Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprot
Autor:
C.A.M.J.J. van den Hondel, C. Konetschny, A.F.J. (Arthur) Ram, C. M. J. Van Zeijl, P.J. Punt, Bernhard Seiboth, M. Lenders, Roy Christiaan Montijn, X.O. Weenink, Christian P. Kubicek
Publikováno v:
Molecular Microbiology. 41:513-525
DNA fragments containing genetic information for five secretion-related small GTPases of Aspergillus niger (srgA-E) were isolated and identified as members of different Rab/Ypt subfamilies. This isolation and the search for similar sequences in funga
Autor:
D. Siedenberg, Ursula Rinas, Mestric Silvija, P.J. Punt, Michael Schmidt, Markus Ganzlin, C.A.M.J.J. van den Hondel
Publikováno v:
Biotechnology Progress. 15:43-50
The first successful expression of the Aequorea victoria green fluorescent protein (GFP) gene in Aspergillus niger is described. When the wild-type GFP gene was expressed in A. niger, neither the fluorescence nor the full translation product of the w
Publikováno v:
Glycoconjugate Journal. 16:99-107
Filamentous fungi are commonly used in the fermentation industry for large scale production of glycoproteins. Several of these proteins can be produced in concentrations up to 20-40 g per litre. The production of heterologous glycoproteins is at leas
Autor:
J. Drint-Kuijvenhoven, C.A.M.J.J. van den Hondel, A. Beijersbergen, P.J. Punt, J. G. M. Hessing, Cornelis Theodorus Verrips, G. M. van Muijlwijk-Harteveld, I. A. van Gemeren
Publikováno v:
Applied Microbiology and Biotechnology, 4, 50, 447-454
The function of the endoplasmic-reticulum-localized chaperone binding protein (BiP) in relation to protein secretion in filamentous fungi was studied. It was shown that the overproduction of several homologous and heterologous recombinant proteins by