Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells

Autor: Alexis Autour, Sunny C. Y. Jeng, Adam D. Cawte, Amir Abdolahzadeh, Angela Galli, Shanker S. S. Panchapakesan, David Rueda, Michael Ryckelynck, Peter J. Unrau

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)

Many aptamer-fluorophore complexes suffer from low quantum yield and low extinction coefficients limiting their usability. Here the authors isolate new Mango aptamers with improved fluorescent properties for both fixed and live-cell imaging by using

Externí odkaz: https://doaj.org/article/ffa23cd158524e5b94c7284e2e879efb

Structure and functional reselection of the Mango-III fluorogenic RNA aptamer

Autor: Robert J. Trachman, Alexis Autour, Sunny C. Y. Jeng, Amir Abdolahzadeh, Alessio Andreoni, Razvan Cojocaru, Ramil Garipov, Elena V. Dolgosheina, Jay R. Knutson, Michael Ryckelynck, Peter J. Unrau, Adrian R. Ferré-D’Amaré

Publikováno v: Nature Chemical Biology
Nature Chemical Biology, Nature Publishing Group, 2019, 15 (5), pp.472-479. ⟨10.1038/s41589-019-0267-9⟩
Nat Chem Biol

International audience; Several turn-on RNA aptamers that activate small molecule fluorophores have been selected in vitro. Among these, the ~30 nucleotide Mango-III is notable because it binds the thiazole orange derivative TO1-Biotin with high affi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf80ed0be14500533faeefb2dd52d27c
https://hal.archives-ouvertes.fr/hal-02112063/document

Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells

Autor: Amir Abdolahzadeh, Adam D. Cawte, Peter J. Unrau, David Rueda, Sunny C. Y. Jeng, Alexis Autour, Angela Galli, Shanker Shyam S. Panchapakesan, Michael Ryckelynck

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2018, 9 (1), ⟨10.1038/s41467-018-02993-8⟩
Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)

Despite having many key roles in cellular biology, directly imaging biologically important RNAs has been hindered by a lack of fluorescent tools equivalent to the fluorescent proteins available to study cellular proteins. Ideal RNA labelling systems

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a062f344e1a553f6d9d386b85e84126f
https://hal-cnrs.archives-ouvertes.fr/hal-02118986

Live cell imaging of genomic loci using fluorescent RNA aptamers

Autor: Peter J. Unrau, David Rueda, Sunny C. Y. Jeng, Adam D. Cawte

Publikováno v: 61st Annual Meeting of the Biophysical-Society
69A

In recent years, there has been an explosion of SELEX-evolved fluorescent RNA aptamers, such as Spinach and Mango, with enhanced folding, fluorescence and a high affinity for their dyes. These aptamers have a drastically improved fluorescence contras

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d41abdc0a5134b5167abc6a010476d3
http://hdl.handle.net/10044/1/53976

RNA complex purification using high-affinity fluorescent RNA aptamer tags

Autor: Shanker Shyam S, Panchapakesan, Sunny C Y, Jeng, Peter J, Unrau

Publikováno v: Annals of the New York Academy of Sciences. 1341

RNA plays important roles in cellular processes, but RNA-protein complexes are notoriously hard to isolate and study. We compare and contrast existing RNA- and protein-purification strategies with the potential of new RNA-tagging systems such as RNA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5d307cd5425f3a59bc99525e2228141b
https://pubmed.ncbi.nlm.nih.gov/25585661