A multi-kingdom genetic barcoding system for precise target clone isolation

Autor: Soh Ishiguro, Kana Ishida, Rina C. Sakata, Hideto Mori, Mamoru Takana, Samuel King, Omar Bashth, Minori Ichiraku, Nanami Masuyama, Ren Takimoto, Yusuke Kijima, Arman Adel, Hiromi Toyoshima, Motoaki Seki, Ju Hee Oh, Anne-Sophie Archambault, Keiji Nishida, Akihiko Kondo, Satoru Kuhara, Hiroyuki Aburatani, Ramon I. Klein Geltink, Yasuhiro Takashima, Nika Shakiba, Nozomu Yachie

Clonal heterogeneity underlies diverse biological processes, including cancer progression, cell differentiation, and microbial evolution. Cell tagging strategies with DNA barcodes have recently enabled analysis of clone size dynamics and clone-restri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::357f721bd143c6e9ba151267c322a171
https://doi.org/10.1101/2023.01.18.524633

Base editors for simultaneous introduction of C-to-T and A-to-G mutations

Autor: Kazuharu Arakawa, Hiroshi Nishimasu, Hiroki Ueda, Rina C. Sakata, Akihiko Kondo, Hideto Mori, Shogo Yamamoto, Motoaki Seki, Kenji Tatsuno, Soh Ishiguro, Masaru Tomita, Mamoru Tanaka, Keiji Nishida, Nanami Masuyama, Hiroyuki Aburatani, Nozomu Yachie, Osamu Nureki

Publikováno v: Nature Biotechnology. 38:865-869

We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c72f2fd67c36164a987e2b2d8175c411
https://doi.org/10.1038/s41587-020-0509-0

Hybrid Strategy for Selecting Compact Set of Clustering Partitions

Autor: Katti Faceli, Vanessa Antunes, Tiemi C. Sakata, Marcilio C. P. de Souto

Publikováno v: Journal of Applied Soft Computing, Springer
Journal of Applied Soft Computing, Springer, 2020, 87, pp.105971. ⟨10.1016/j.asoc.2019.105971⟩

International audience; The selection of the most appropriate clustering algorithm is not a straightforward task, given that there is no clustering algorithm capable of determining the actual groups present in any dataset. A potential solution is to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad5b03a6ea42ab945e2fe788c3cb763d
https://hal.archives-ouvertes.fr/hal-02388417

Educational games and the new forms of interactions

Autor: Luciana A. M. Zaina, Suellen Martinelli, Elisa Marques de Castro, Tiemi C. Sakata

Publikováno v: Smart Learning Environments. 6

Games are seen as environments that promote the engagement of children to the learning of Computational Thinking (CT) concepts. The new forms of interaction using body movements and gestures have increased in particular in video-games. However, few s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5a12b988c733ba34020deb5777b94da4
https://doi.org/10.1186/s40561-019-0099-9

Playing a Computational Thinking Game using Hand Gestures

Autor: Tiemi C. Sakata, Luciana A. M. Zaina, Elisa Marques de Castro

Publikováno v: ICALT

The concern on promoting the development of Computational Thinking (CT) in children has increased over the years. Games are recognized as a good strategy for presenting CT and for engaging children to the learning of its concepts. Hand gestures are a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f35e3d56893c71ef02b995a0db44cd4d
https://doi.org/10.1109/icalt.2019.00029