Single-cell measurements of enzyme levels as a predictive tool for cellular fates during organic acid production

Autor: C. Gustavo Pesce, Laura Ruohonen, Merja Penttilä, Stefan Zdraljevic, Jussi Jäntti, Drew T. Wagner, Kevin Cheng, Orna Resnekov

Publikováno v: Zdraljevic, S, Wagner, D, Cheng, K, Ruohonen, L, Jäntti, J, Penttilä, M, Resnekov, O & Pesce, C G 2013, ' Single-cell measurements of enzyme levels as a predictive tool for cellular fates during organic acid production ', Applied and Environmental Microbiology, vol. 79, no. 24, pp. 7569-7582 . https://doi.org/10.1128/AEM.01749-13

Organic acids derived from engineered microbes can replace fossil-derived chemicals in many applications. Fungal hosts are preferred for organic acid production because they tolerate lignocellulosic hydrolysates and low pH, allowing economic producti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb9824ad8bb9abb0a2800615270f3a84
https://cris.vtt.fi/en/publications/52a9f54d-23b9-40a0-840c-2af0b4b058e7

Negative feedback that improves information transmission in yeast signalling

Autor: C. Gustavo Pesce, Eduard Serra, Kirsten R. Benjamin, Mark R. Holl, Andrew S. Gordon, Larry Lok, Alejandro Colman-Lerner, David Pincus, Roger Brent, Richard C. Yu

Publikováno v: Nature. 456:755-761

Haploid Saccharomyces cerevisiae yeast cells use a prototypic cell signalling system to transmit information about the extracellular concentration of mating pheromone secreted by potential mating partners. The ability of cells to respond distinguisha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f2cdc6b0f5d8b990d3bfab32959f83ec
https://doi.org/10.1038/nature07513

Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae

Autor: Marilyn G. Wiebe, Mervi Toivari, Dominik Mojzita, C. Gustavo Pesce, Laura Ruohonen, Hannu Maaheimo, Yvonne Nygård, Orna Resnekov, Merja Penttilä

Publikováno v: Nygård, Y, Maaheimo, H, Mojzita, D, Toivari, M, Wiebe, M, Resnekov, O, Gustavo Pesce, C, Ruohonen, L & Penttilä, M 2014, ' Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae ', Metabolic Engineering, vol. 25, pp. 238-247 . https://doi.org/10.1016/j.ymben.2014.07.005

D-xylonate is a potential platform chemical which can be produced by engineered Saccharomyces cerevisiae strains. In order to address production constraints in more detail, we analysed the role of lactone ring opening in single cells and populations.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6aba655e8a10555df274744449c3e212
https://pubmed.ncbi.nlm.nih.gov/25073011

The CCAAT-binding Proteins CP1 and NF-I Cooperate with ATF-2 in the Transcription of the Fibronectin Gene

Autor: C. Gustavo Pesce, Claudio R. Alonso, Alberto R. Kornblihtt

Publikováno v: Journal of Biological Chemistry. 271:22271-22279

We have previously proposed a molecular interaction between the liver factors that bind to the cyclic AMP response element (CRE) and CCAAT sites of the fibronectin (FN) gene based on the following evidence: (i) the close spacing of 20 base pairs betw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97703d8286f33ff2a0877f86ff13c219
https://doi.org/10.1074/jbc.271.36.22271

Erratum: Regulated cell-to-cell variation in a cell-fate decision system

Autor: Alejandro Colman-Lerner, C. Gustavo Pesce, Andrew S. Gordon, Roger Brent, Eduard Serra, Orna Resnekov, Drew Endy, Tina Chin

Publikováno v: Nature. 439:502-502

Nature 437, 699–706 (2005) In Fig. 1b of this Article, the x axis of the right-hand plot should be labelled 'α-Factor system output in each cell (CFP F.U. × 106)' and not 'ACT1 system output in each cell (CFP F.U. × 105)'. In addition, Supplemen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9f5ebf82ac7668516022aa19bb8db9a3
https://doi.org/10.1038/nature04476