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Autor:
B. Cambou, Yves Lelièvre, C. Giuliacci, Terence Cartwright, G. Jung, A. Dugué, Fabienne Parker, J. Boiziau, J.-F. Mayaux, Ph.-H. Hirel, D. Outerovitch, C. Peltiers, R. Boulay
Publikováno v:
Antiviral Chemistry and Chemotherapy. 1:9-15
The 99-amino-acid HIV-1 aspartic proteinase was expressed to high levels in Escherichia coli using a T7 expression system. About 50% of the insoluble material after sonication of the bacteria was composed of aggregated proteinase. Subsequent renatura