Zobrazeno 1 - 10
of 18
pro vyhledávání: '"C. Ganassin"'
Publikováno v:
Fisheries Research. 82:290-303
Risk assessment has become a key part of management plans for wild capture fisheries. However, for data-deficient fisheries and those with limited knowledge of ecological interactions a qualitative risk assessment method is needed. This paper present
Autor:
Niels C. Bols, Keith R. Solomon, Joanne L. Parrott, Audrey Gamble, Niel A. Karrow, Rosemarie C. Ganassin, Herman J. Boermans, Donald T. Bennie, D. George Dixon, James P. Sherry
Publikováno v:
Journal of Great Lakes Research. 29:280-295
Our objective was to assess whether or not exposure to sites within Hamilton Harbour known to be highly contaminated with polycyclic aromatic hydrocarbons (PAHs), heavy metals, and sewage treatment plant (STP) effluent could affect macrophage functio
Autor:
Rosemarie C. Ganassin, Niels C. Bols
Publikováno v:
Methods in Cell Science. 22:147-152
A technique for the clonal culture of rainbow trout leukocytes in a methylcellulose matrix can be used to identify growth factors and other substances affecting cell proliferation and development in fish. Methylcellulose supports colony formation by
Publikováno v:
Cell Biology and Toxicology. 15:299-309
A cell line, PHL, has been successfully established from newly hatched herring larvae. The cells are maintained in growth medium consisting of Leibovitz's L-15 supplemented with 15% fetal bovine serum (FBS), and have been cryopreserved and maintain v
Autor:
Niels C. Bols, Rosemarie C. Ganassin
Publikováno v:
Fish & Shellfish Immunology. 8:457-476
A new rainbow trout cell line, RTS11, arose spontaneously from a long-term haemopoietic culture of spleen. For routine subculture, RTS11 requires 20–30% fetal bovine serum, a high cell density, and conditioned medium from previous cultures. Through
Autor:
Rosemarie C. Ganassin, Niels C. Bols
Publikováno v:
Fish & Shellfish Immunology. 6:17-34
Different tissue dissociation protocols and serum supplements resulted in a variety of primary cultures from rainbow trout spleens. These were classified as either haemopoietic or non-haemopoietic. In haemopoietic cultures, a complex stromal layer de
Publikováno v:
Journal of Tissue Culture Methods. 16:133-142
The use of a DNA-binding dye, bisbenzimidazole (H33258), and a microplate fluorometer, CytoFluor 2350, was optimized to measure cell number in Chinook salmon embryo cell cultures (CHSE-214). The uniformity of cell homogenates, which were prepared pri
Publikováno v:
Cytotechnology. 16:159-166
The glutamine requirement for the in vitro proliferation of fish cells was investigated with cell lines from four different species and three tissues: goldfish skin (GFSk-S1), Chinook salmon embryo (CHSE-214), and rainbow trout liver (RTL-W1) and spl
Publikováno v:
Cell Biology International. 17:677-684
Chicken ovalbumin and bovine serum albumin (BSA) were compared as supplements to the basal medium, L-15, for the serum-free cultivation of the Chinook Salmon Embryo cell line, CHSE-214. Unlike L-15 alone, ovalbumin and some commercial BSA preparation
Autor:
Niels C. Bols, R. C. Ganassin
Publikováno v:
Cytotechnology. 8:21-29
The Chinook salmon embryo cell line, CHSE-214, grew well in fetal bovine serum (FBS) but poorly in dialyzed (d) FBS. Purines restored most but not all growth-promoting activity to dFBS, which suggests that purines account for a large portion of the d