Zobrazeno 1 - 10 of 36 pro vyhledávání: '"C. Flachmeier"'
1
High-sensitivity protein detection by a new 'contact-copy' method using a protein A-neomycin phosphotransferase II fusion protein
Autor: Ch. Coutelle, C. Flachmeier, G. Schmidt, G. Behrendt, H.-D. Hunger
Publikováno v: Analytical Biochemistry. 186:159-164
A new system for high-sensitivity protein detection by an immunoenzymatic “contact-copy” procedure is described. It is based on two components: (i) a microbiologically produced bifunctional fusion protein of protein A and neomycin phosphotransfer
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7b46579210141a92f1a889ba7c6bcd1
https://doi.org/10.1016/0003-2697(90)90590-6
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3
Quantitative western blotting using [gamma-33P]ATP and the ultrasensitive bio-imaging analyzer
Autor: H.-D. Hunger, G. Schmidt, C. Flachmeier
Publikováno v: Analytical biochemistry. 217(1)
This report describes a method for simultaneous quantitative determination of antigenes from protein blots based on the highly sensitive "contact-copy" procedure, applying the phosphorylation reaction as a general detection principle. Using ultrasens
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdc2a5aedc5e26de61ba8c16a4cf8a65
https://pubmed.ncbi.nlm.nih.gov/8203743
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4
Ultrasensitive enzymatic radioimmunoassay using a fusion protein of protein A and neomycin phosphotransferase II in two-chamber-well microtiter plates
Autor: G. Schmidt, C. Flachmeier, G. Behrendt, H.-D. Hunger, Ch. Coutelle
Publikováno v: Analytical biochemistry. 187(1)
A new sensitive method for antigen detection employing a phosphorylation reaction is described using human serum albumin as a model. The antigen is initially bound to the surface of polystyrene microtiter plates and reacted with an antibody (rabbit).
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52c3ab9f0260006138e809c2425deb37
https://pubmed.ncbi.nlm.nih.gov/2164793
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5
Use of cyanuric chloride-activated paper for detection of subpicogram quantities of specific DNA sequences and its application to linked restriction fragment length polymorphism analysis in a Duchenne muscular dystrophy affected family
Autor: H.-D. Hunger, Ch. Coutelle, A. Speer, C. Flachmeier, R. Hanke, G. Behrendt
Publikováno v: Analytical Biochemistry. 165:45-55
Conditions for the optimal use of cyanuric chloride-activated (CCA) paper in Southern transfer hybridization experiments of genomic DNA were investigated. They depend critically on pH and ionic strength during transfer and on the composition of the h
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7eda9f42992274b59afeb9fad1399cce
https://doi.org/10.1016/0003-2697(87)90199-0
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6
Akademický článek
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7
Machiavellianism, External Control, and Cognitive Style of American and West German CO-EDS
Autor: L. C. Flachmeier, Georgette K. Maroldo
Publikováno v: Psychological Reports. 42:1315-1317
This study concerned similarities and differences between 59 American and 97 West German co-eds with respect to Machiavellianism, external control, and cognitive style. American and West German co-eds differed in Machiavellian orientation and externa
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1bb4864fc2a387f653fdb2fb3bf54fb4
https://doi.org/10.2466/pr0.1978.42.3c.1315
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8
A new neomycin phosphotransferase II solid phase assay in combination with polyacrylamide sodium dodecylsulphate gel electrophoresis
Autor: Ch. Coutelle, H.-D. Hunger, D. Merkel, G. Schmidt, G. Behrendt, C. Flachmeier
Publikováno v: Journal of biochemical and biophysical methods. 18(4)
A new general method for the determination of neomycin phosphotransferase (NPT) II (EC 2.7.1.95) activity in cell extracts after separation in SDS-polyacrylamide gels is described. The enzymatic activity of NPT II is restored after SDS-polyacrylamide
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b21ced383e6f25db89b2fd0d3a4dddd3
https://pubmed.ncbi.nlm.nih.gov/2550536
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9
A protein-free blocking system for the covalently binding matrix cyanuric chloride-activated paper in immunological procedures
Autor: G. Schmidt, G. Behrendt, Ch. Coutelle, H.-D. Hunger, C. Flachmeier
Publikováno v: Journal of biochemical and biophysical methods. 17(1)
A new protein-free blocking system containing 10% (w/v) sulfanilic acid/10% (v/v) triethanolamine/0.05% (v/v) Tween 20 has been used to block free binding sites of the covalently binding matrix cyanuric chloride-activated paper (CCA-paper). This meth
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf8a607e9d611c2d47fb996243bffd09
https://pubmed.ncbi.nlm.nih.gov/3235766
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10
Relationship between Machiavellianism, External Control, and Cognitive Style among College Students
Autor: K. L. Russell, L. K. Johnston, L. C. Flachmeier, E. J. Reitan, M. I. Peter, Georgette K. Maroldo, J. L. Mayer
Publikováno v: Psychological Reports. 39:805-806
This experiment determined relationships between Machiavellianism, locus of control, and cognitive style. 115 subjects, 56 males, 59 females, ages 18 to 21, were selected from Interim students at Texas Lutheran College, Seguin, Texas. The M Mach IV t
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2199703bb8e8fc155bb34c2a3c0c67eb
https://doi.org/10.2466/pr0.1976.39.3.805
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