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pro vyhledávání: '"C. Eric Hodgman"'
Publikováno v:
Synthetic and Systems Biotechnology, Vol 1, Iss 1, Pp 2-6 (2016)
Cell-free protein synthesis (CFPS) systems from crude lysates have benefitted from modifications to their enzyme composition. For example, functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein synthesis y
Externí odkaz:
https://doaj.org/article/5948679f5f4d4035be54a5f6225c725e
Publikováno v:
Synthetic and Systems Biotechnology, Vol 1, Iss 1, Pp 2-6 (2016)
Synthetic and Systems Biotechnology
Synthetic and Systems Biotechnology
Cell-free protein synthesis (CFPS) systems from crude lysates have benefitted from modifications to their enzyme composition. For example, functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein synthesis y
Publikováno v:
Biochemical Engineering Journal. 91:140-148
Saccharomyces cerevisiae is a promising source organism for the development of a practical, eukaryotic crude extract based cell-free protein synthesis (CFPS) system. Crude extract CFPS systems represent a snapshot of the active metabolism in vivo, in
Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis
Autor:
C. Eric Hodgman, Michael C. Jewett
Publikováno v:
New Biotechnology. 31:499-505
Eukaryotic cell-free protein synthesis (CFPS) systems are limited, in part, by inefficient translation initiation. Here, we report three internal ribosome entry site (IRES) sequences from the Dicistroviridae family that are highly active in yeast CFP
Autor:
Michael C. Jewett, C. Eric Hodgman
Publikováno v:
Biotechnology and Bioengineering. 110:2643-2654
Cell-free protein synthesis (CFPS) has emergedasapowerfulplatformtechnologytohelpsatisfythegrowingdemand for simple, affordable, and efficient proteinproduction.Inthisarticle,wedescribeanovelCFPSplatformderived from the popular bio-manufacturing org
Publikováno v:
Biotechnology Advances. 30:1185-1194
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and trans
Autor:
Michael C. Jewett, C. Eric Hodgman
Publikováno v:
Metabolic Engineering. 14:261-269
Cell-free synthetic biology is emerging as a powerful technology aimed to understand, harness, and expand the capabilities of natural biological systems without using intact cells. Cell-free systems bypass cell walls and remove genetic regulation to
Autor:
C Eric, Hodgman, Michael C, Jewett
Publikováno v:
Biotechnology and bioengineering. 110(10)
Cell-free protein synthesis (CFPS) has emerged as a powerful platform technology to help satisfy the growing demand for simple, affordable, and efficient protein production. In this article, we describe a novel CFPS platform derived from the popular
Publikováno v:
FEBS Letters. (15):1723-1727
Eukaryotic cell-free protein synthesis (CFPS) is limited by the dependence on costly high-energy phosphate compounds and exogenous enzymes to power protein synthesis (e.g., creatine phosphate and creatine kinase, CrP/CrK). Here, we report the ability