Výsledky vyhledávání - "C. C. M. Huijbregts"

Characterization of recombinant C1 inhibitor P1 variants

Autor: C E Hack, C. C. M. Huijbregts, Eric Eldering, Colin Longstaff, Y T Lubbers

Publikováno v: Journal of biological chemistry, 267(10), 7013-7020. American Society for Biochemistry and Molecular Biology Inc.

Twelve human C1 inhibitor P1 variants were constructed by site-directed mutagenesis of the codon for arginine 444 and were expressed in COS-1 cells to analyze the functional properties. The ability to bind to target proteases, as well as potential su

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb209325cda8500ad6b5771fdec6516a
https://doi.org/10.1016/s0021-9258(19)50529-4

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A Novel Sensitive Assay for Functional Factor XII Based on the Generation of Kallikrein-C1-lnhibitor Complexes in Factor Xll-Deficient Plasma by Glass-Bound Factor XII

Autor: J. H. Nuijens, C. C. M. Huijbregts, C. E. Hack, D. M. Dors

Publikováno v: Thrombosis and Haemostasis. 67:644-648

SummaryWe developed a very sensitive assay for functional factor XII. This assay is based on the property of glass-bound factor XII to activate prekallikrein (PK) into kallikrein in factor XH-deficient plasma, which is assessed by measuring the forma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a6f0b6565823739cf5a3cde845cebc5e
https://doi.org/10.1055/s-0038-1648516

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Quantification of Functional and Inactivated α2-Macroglobulin in Sepsis

Autor: J. J. Abbink, Strack van Schijndel Rj, A. J. M. Eerenberg, C. E. Hack, J. H. Nuijens, Lambertus G. Thijs, C. C. M. Huijbregts

Publikováno v: Thrombosis and Haemostasis. 65:032-039

Alpha 2-macroglobulin (alpha 2 M) in vitro inhibits numerous proteinases that are generated during inflammatory reactions and therefore, probably plays an important role in diseases such as sepsis. To monitor the state of alpha 2 M in sepsis, we deve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::95589fae9d85c5009612b442beaca53a
https://doi.org/10.1055/s-0038-1647450

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Detection of Activation of the Contact System of Coagulation In Vitro and In Vivo: Quantitation of Activated Hageman Factor-C1-Inhibitor and Kallikrein-C1-Inhibitor Complexes by Specific Radioimmunoassays

Autor: J. H. Nuijens, C. E. Hack, A. J. M. Eerenberg, M. Cohen, C. C. M. Huijbregts, A.C. de Vries, G. O. Navis, J. C. Bakker

Publikováno v: Thrombosis and Haemostasis. 58:778-785

SummaryRadioimmunoassays (RIAs) for the detection of C1-inhihitor (C1-Inh) complexed to either kallikrein or activated Hageman factor (factor XIIa) are described. Kallikrein-C1-Inh or factor XIIa-C1-Inh complexes were bound to Scpharosc to which mono

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::32e7f9a17e57b5b01cbfea60cdeb9ed0
https://doi.org/10.1055/s-0038-1645969

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Quantification of plasma factor XIIa-Cl(-)-inhibitor and kallikrein-Cl(- )-inhibitor complexes in sepsis

Autor: A. J. M. Eerenberg-Belmer, J. H. Nuijens, C. C. M. Huijbregts, R.J.M. Strack van Schijndel, J. J. Abbink, C. E. Hack, L. G. Thijs, R. J. F. Felt-Bersma

Publikováno v: Blood. 72:1841-1848

Considerable evidence indicates that activation of the contact system of intrinsic coagulation plays a role in the pathogenesis of septic shock. To monitor contact activation in patients with sepsis, we developed highly sensitive radioimmunoassays (R

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3c0c22fdccd9f0655708b2ba7eee4b4a
https://doi.org/10.1182/blood.v72.6.1841.1841

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Influence of ionic strength, EDTA concentration, endogenous C1q and polyanions on the 125I-C1q-binding test

Autor: C. C. M. Huijbregts, J. Paardekooper, C. E. Hack

Publikováno v: Journal of Immunological Methods. 72:197-206

Several parameters of the 125I-C1q-binding test were investigated: ionic strength, pH, concentration of EDTA, influence of serum C1q and the possibility of interference by polyanions. Lowering the ionic strength of the borate buffer resulted in incre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c59349cd52a9f11061f75a88a438960
https://doi.org/10.1016/0022-1759(84)90448-4

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DETECTION OF INACTIVATED α2-MACROGLOBULIN (α2M) IN HUMAN PLASMA USING MONOCLONAL ANTIBODIES

Autor: J. H. Nuijens, J. J. Abbink, E Hack, C. C. M. Huijbregts

Publikováno v: XIth International Congress on Thrombosis and Haemostasis.

Monoclonal antibodies (mAbs) were raised against human a2M. Five mAbs that bound to α2M in ELISA were further analyzed by a radioimmunoassay (RIA) for their reaction with three types of α2M: native α2M, chemically inactivated α2M (iα2M) (methyla

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::173bb5764f1d39dfff79a47c704f723f
https://doi.org/10.1055/s-0038-1643865

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