Zobrazeno 1 - 10
of 17
pro vyhledávání: '"C T, Wittwer"'
Publikováno v:
Clinical chemistry. 47(5)
Molecular detection methods for HER2/neu gene amplification include fluorescence in situ hybridization (FISH) and competitive PCR. We designed a quantitative PCR system utilizing fluorescent hybridization probes and a competitor that differed from th
Publikováno v:
Clinical chemistry. 46(3)
Autor:
P S, Bernard, C T, Wittwer
Publikováno v:
Clinical chemistry. 46(2)
Autor:
M J, Lay, C T, Wittwer
Publikováno v:
Clinical chemistry. 43(12)
A single-step method for factor V Leiden genotyping is presented that uses rapid-cycle PCR and simultaneous fluorescence analysis with resonance energy transfer probes. A fragment of the factor V gene containing the mutation is amplified asymmetrical
Publikováno v:
Blood. 88(2)
A 10-year-old girl with paroxysmal nocturnal hemoglobinuria (PNH) received an infusion of syngeneic bone marrow without preparative marrow ablation or immunosuppression. Following transplant, the patient became asymptomatic in concordance with an inc
Autor:
S R, Florell, J J, Townsend, E C, Klatt, T J, Pysher, C M, Coffin, C T, Wittwer, D H, Viskochil
Publikováno v:
American journal of medical genetics. 63(4)
Aprosencephaly is a rare, lethal malformation sequence of the central nervous system that has been attributed to a postneuralation encephaloclastic process. We describe autopsy findings consistent with aprosencephaly in 2 fetuses conceived from a con
Publikováno v:
Clinical chemistry. 39(5)
Rapid cycle DNA amplification is a polymerase chain reaction technique with improved product specificity and cycle times of 20-60 s, allowing complete 30-cycle reactions in 10-30 min. The presence or absence of the delta F508 deletion and wild-type a
Publikováno v:
The American journal of pathology. 141(6)
Alternatives to Southern blot hybridization for gene rearrangement analysis are being studied because of the time, labor, cost, and radioisotopes required for this technique. We have utilized a rapid, hot air, thermocycling polymerase chain reaction
Publikováno v:
Oncology research. 4(4-5)
Western blotting experiments with topoisomerase II antiserum were performed to measure type II topoisomerase content in human tissues. In this study, antiserum produced against the C-terminus of recombinant HeLa topoisomerase II recognized both p170
Autor:
J B, O'Connell, D G, Renlund, E H, Hammond, C T, Wittwer, R L, Yowell, C W, DeWitt, K W, Jones, W A, Gay, R L, Menlove, M R, Bristow
Publikováno v:
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation. 10(2)
Because administration of murine monoclonal anti-CD3 antibody (OKT3) may result in the formation of human antimouse antibody, which complexes with OKT3, we conducted this study to assess the incidence and effect of human antimouse antibody formation