A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research.

Autor: Suzannah J Rihn, Andres Merits, Siddharth Bakshi, Matthew L Turnbull, Arthur Wickenhagen, Akira J T Alexander, Carla Baillie, Benjamin Brennan, Fiona Brown, Kirstyn Brunker, Steven R Bryden, Kerry A Burness, Stephen Carmichael, Sarah J Cole, Vanessa M Cowton, Paul Davies, Chris Davis, Giuditta De Lorenzo, Claire L Donald, Mark Dorward, James I Dunlop, Matthew Elliott, Mazigh Fares, Ana da Silva Filipe, Joseph R Freitas, Wilhelm Furnon, Rommel J Gestuveo, Anna Geyer, Daniel Giesel, Daniel M Goldfarb, Nicola Goodman, Rory Gunson, C James Hastie, Vanessa Herder, Joseph Hughes, Clare Johnson, Natasha Johnson, Alain Kohl, Karen Kerr, Hannah Leech, Laura Sandra Lello, Kathy Li, Gauthier Lieber, Xiang Liu, Rajendra Lingala, Colin Loney, Daniel Mair, Marion J McElwee, Steven McFarlane, Jenna Nichols, Kyriaki Nomikou, Anne Orr, Richard J Orton, Massimo Palmarini, Yasmin A Parr, Rute Maria Pinto, Samantha Raggett, Elaine Reid, David L Robertson, Jamie Royle, Natalia Cameron-Ruiz, James G Shepherd, Katherine Smollett, Douglas G Stewart, Meredith Stewart, Elena Sugrue, Agnieszka M Szemiel, Aislynn Taggart, Emma C Thomson, Lily Tong, Leah S Torrie, Rachel Toth, Margus Varjak, Sainan Wang, Stuart G Wilkinson, Paul G Wyatt, Eva Zusinaite, Dario R Alessi, Arvind H Patel, Ali Zaid, Sam J Wilson, Suresh Mahalingam

Publikováno v: PLoS Biology, Vol 19, Iss 2, p e3001091 (2021)

The recent emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the underlying cause of Coronavirus Disease 2019 (COVID-19), has led to a worldwide pandemic causing substantial morbidity, mortality, and economic devastation. In

Externí odkaz: https://doaj.org/article/cc007f107e3a422ab1bfef32714d1945

Expression of Concern: Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs

Autor: Graeme K. Carnegie, Judith E. Sleeman, Nick Morrice, C. James Hastie, Mark W. Peggie, Amanda Philp, Angus I. Lamond, Patricia T. W. Cohen

Publikováno v: Journal of cell science. 135(19)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25381fbed8764bd4a6ad71028ed5d6c4
https://pubmed.ncbi.nlm.nih.gov/12668731

Elaboration of a MALDI-TOF Mass Spectrometry-based Assay of Parkin Activity and High-Throughput screening platform for Parkin Activators

Autor: Ryan Traynor, Jennifer Moran, Michael Stevens, Axel Knebel, Bahareh Behrouz, Kalpana Merchant, C. James Hastie, Paul Davies, Miratul M. K. Muqit, Virginia De Cesare

Parkinson’s disease (PD) is a progressive neurological disorder that manifests clinically as alterations in movement (bradykinesia, postural instability, loss of balance, and resting tremors) as well as multiple non-motor symptoms including but not

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::860152ff160f8865fe516b08cc35f28b
https://doi.org/10.1101/2022.03.04.482851

Activity-based probe profiling of RNF12 E3 ubiquitin ligase function in Tonne-Kalscheuer syndrome

Autor: Francisco Bustos, Sunil Mathur, Carmen Espejo-Serrano, Rachel Toth, C James Hastie, Satpal Virdee, Greg M Findlay

Publikováno v: Life Science Alliance. 5:e202101248

Ubiquitylation enzymes are involved in all aspects of eukaryotic biology and are frequently disrupted in disease. One example is the E3 ubiquitin ligase RNF12/RLIM, which is mutated in the developmental disorder Tønne-Kalscheuer syndrome (TOKAS). RN

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44c1c79f75533deada6abd4d40918b33
https://doi.org/10.26508/lsa.202101248

High-throughput matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry-based deubiquitylating enzyme assay for drug discovery

Autor: Virginia, De Cesare, Jennifer, Moran, Ryan, Traynor, Axel, Knebel, Maria Stella, Ritorto, Matthias, Trost, Hilary, McLauchlan, C James, Hastie, Paul, Davies

Publikováno v: Nature protocols. 15(12)

Deubiquitylating enzymes (DUBs) play a vital role in the ubiquitin pathway by editing or removing ubiquitin from their substrate. As breakthroughs within the ubiquitin field continue to highlight the potential of deubiquitylating enzymes as drug targ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::66f7e16d8d56c29b82f0a3df02e56481
https://pubmed.ncbi.nlm.nih.gov/33139956