Výsledky vyhledávání - "C J, Unkefer"

CN- secondary ions form by recombination as demonstrated using multi-isotope mass spectrometry of 13C- and 15N-labeled polyglycine

Autor: Gregory McMahon, C. J. Unkefer, H. Francois Saint-Cyr, Claude Lechene

Publikováno v: Journal of the American Society for Mass Spectrometry. 17(8)

We have studied the mechanism of formation CN− secondary ions under Cs+ primary ion bombardment. We have synthesized 13C and 15N labeled polyglycine samples with the distance between the two labels and the local atomic environment of the 13C label

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca40c4e098c3da51033e368b93e12a9b
https://pubmed.ncbi.nlm.nih.gov/16750387

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Relationship of exo-beta-D-galactofuranosidase kinetic parameters to the number of phosphodiesters in Penicillium fellutanum peptidophosphogalactomannan: enzyme purification and kinetics of glycopeptide and galactofuran chain hydrolysis

Autor: B A, Tuekam, Y I, Park, C J, Unkefer, J E, Gander

Publikováno v: Applied and environmental microbiology. 67(10)

Extracellular Penicillium fellutanum exo-beta-D-galactofuranosidase, with a mass of 70 kDa, was purified to apparent homogeneity. The enzyme was used to investigate the influence of phosphodiesters of the peptidophosphogalactomannans pP(2)GM(ii) and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2edd3844d77129e5e8c583d7a60d8d90
https://pubmed.ncbi.nlm.nih.gov/11571168

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Biogenesis of pyrroloquinoline quinone from 3C-labeled tyrosine

Autor: C J, Unkefer, D R, Houck, B M, Britt, T R, Sosnick, J L, Hanners

Publikováno v: Methods in enzymology. 258

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::722fb03d9c951fecb9f6b5c67f835c3b
https://pubmed.ncbi.nlm.nih.gov/8524153

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13C and 15N nuclear magnetic resonance evidence of the ionization state of substrates bound to bovine dihydrofolate reductase

Autor: B S, Selinsky, M E, Perlman, R E, London, C J, Unkefer, J, Mitchell, R L, Blakley

Publikováno v: Biochemistry. 29(5)

The state of protonation of substrates bound to mammalian dihydrofolate reductase (DHFR) has significance for the mechanism of catalysis. To investigate this, dihydrofolate and dihydropteroyl-pentaglutamate have been synthesized with 15N enrichment a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e445c48315c2f2f60f792a84d8e78037
https://pubmed.ncbi.nlm.nih.gov/2322563

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The 5-O-beta-D-galactofuranosyl-containing peptidophosphogalactomannan of Penicillium charlesii. Characterization of the mannan by 13C NMR spectroscopy

Autor: C J, Unkefer, J E, Gander

Publikováno v: The Journal of biological chemistry. 265(2)

Penicillium charlesii secretes a galactofuranosyl and phosphodiester-containing peptidophosphogalactomannan (pPGM). A linear mannan was prepared from pPGM by treatment with 48% aqueous HF which selectively cleaves galactofuranosyl and phosphodiesters

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::bb7c11fb6ff572332825545b4396c0b9
https://pubmed.ncbi.nlm.nih.gov/2136849

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The biosynthesis of tabtoxinine-beta-lactam. Use of specifically 13C-labeled glucose and 13C NMR spectroscopy to identify its biosynthetic precursors

Autor: Richard D. Durbin, R E London, T F Uchytil, Pat J. Langston-Unkefer, C J Unkefer

Publikováno v: Journal of Biological Chemistry. 262:4994-4999

Tabtoxinine-beta-lactam, an irreversible inhibitor of glutamine synthetase is produced by several pathovars of Pseudomonas syringae. We have examined tabtoxinine-beta-lactam biosynthesis, an important and poorly characterized step in pathogenesis cau

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5e9038ee2e47af06fa85d373bca1b410
https://doi.org/10.1016/s0021-9258(18)61144-5

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The 5-O-beta-D-galactofuranosyl-containing glycopeptide from Penicillium charlesii. Identification of phosphocholine attached to C-6 of mannopyranosyl residues of the mannan region

Autor: C Jackson, J E Gander, C J Unkefer

Publikováno v: Journal of Biological Chemistry. 257:2491-2497

Peptidophosphogalactomannan from Penicillium charlesii contains ethanolamine, presumably linked via phosphodiester to galactomannan (Rick, P. D., Drewes, L. R., and Gander, J. E., (1974) J. Biol. Chem. 249, 2072-2078). These investigations have been

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::45e809540f087e134f52687495f9d71a
https://doi.org/10.1016/s0021-9258(18)34950-0

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The 5-O-beta-D-galactofuranosyl-containing glycopeptide from Penicillium charlesii. Carbon 13 nuclear magnetic resonance studies

Autor: C J Unkefer, J E Gander

Publikováno v: Journal of Biological Chemistry. 254:12131-12135

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e75ba70510cb40e6d34f6d4672f4efbc
https://doi.org/10.1016/s0021-9258(19)86439-6

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In vivo studies of pyridine nucleotide metabolism in Escherichia coli and Saccharomyces cerevisiae by carbon-13 NMR spectroscopy

Autor: C J Unkefer, R E London

Publikováno v: Journal of Biological Chemistry. 259:2311-2320

Pyridine nucleotide metabolism has been studied in vivo in a prokaryotic (Escherichia coli) and a eukaryotic (Saccharomyces cerevisiae) system cultured in a medium containing carbon-13-labeled nicotinic acid, followed by NMR detection of the labeled

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::094afbad30921f512684ec8ab7186f77
https://doi.org/10.1016/s0021-9258(17)43354-0

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