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pro vyhledávání: '"C G Pritchard"'
Publikováno v:
Clinical chemistry. 43(9)
Point-of-care (POC) coagulation tests with paramagnetic iron oxide particles have provided alternatives to testing previously done only in the laboratory. With this technology, POC fibrinolytic tests have followed quietly the trail blazed by POC clot
Autor:
C G, Pritchard, J E, Stefano
Publikováno v:
Annales de biologie clinique. 48(7)
A sensitive, amplified assay for HIV-1 pol region RNA was developed using RNA probes which are replicated by the RNA-dependent RNA polymerase, Q beta replicase. A synthetic target RNA was hybridized in cell lysates prepared with guanidine thiocyanate
Publikováno v:
Journal of Biological Chemistry. 258:9810-9819
Most, if not all, of the DNA polymerase alpha activity in monkey and human cells was complexed with at least two proteins, C1 and C2, that together stimulated the activity of this enzyme from 180- to 1800-fold on low concentrations of denatured DNA,
Autor:
M L DePamphilis, C G Pritchard
Publikováno v:
Journal of Biological Chemistry. 258:9801-9809
Essentially all of the DNA polymerase alpha activity in CV-1 monkey cells could be extracted as an enzyme complex that used DNA substrates with a low primer:template ratio, such as denatured DNA, at least 25 times more efficiently than did purified a
Publikováno v:
The Journal of biological chemistry. 258(16)
Most, if not all, of the DNA polymerase alpha activity in monkey and human cells was complexed with at least two proteins, C1 and C2, that together stimulated the activity of this enzyme from 180- to 1800-fold on low concentrations of denatured DNA,
Publikováno v:
Clinical chemistry. 35(9)
Amplifiable hybridization probes--molecules with a probe sequence embedded within the sequence of a replicatable RNA--will promote the development of sensitive clinical assays. To demonstrate their utility, we prepared a recombinant RNA that containe
Autor:
C G, Pritchard, M L, DePamphilis
Publikováno v:
The Journal of biological chemistry. 258(16)
Essentially all of the DNA polymerase alpha activity in CV-1 monkey cells could be extracted as an enzyme complex that used DNA substrates with a low primer:template ratio, such as denatured DNA, at least 25 times more efficiently than did purified a