Zobrazeno 1 - 10
of 17
pro vyhledávání: '"C D, de Gooijer"'
Autor:
R C, Dorresteijn, G, Wieten, P T, van Santen, M C, Philippi, C D, de Gooijer, J, Tramper, E C, Beuvery
Publikováno v:
Cytotechnology. 23(1-3)
The production of biologicals is subject to strict governmental regulations. These are drawn up in current good manufacturing practices (cGMP), a.o. by the U.S. Food and Drug Administration. To implement cGMP in a production facility, plant automatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::89b24dc07afa134edddfc2b89c06c2ac
https://pubmed.ncbi.nlm.nih.gov/22358517
https://pubmed.ncbi.nlm.nih.gov/22358517
Autor:
J J, van der Pol, M, Machnik, M, Biselli, T, Portela-Klein, C D, de Gooijer, J, Tramper, C, Wandrey
Publikováno v:
Cytotechnology. 24(1)
The monoclonal-antibody production of an immobilized hybridoma cell line cultivated in a fluidized-bed reactor was monitored on-line for nearly 900 h. The monoclonal antibody concentration was determined by an immuno affinity-chromatography method (A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bb4ea5a95391b54904a9e56e54913e67
https://pubmed.ncbi.nlm.nih.gov/22358593
https://pubmed.ncbi.nlm.nih.gov/22358593
Publikováno v:
Cytotechnology. 32(2)
Hybridoma cells were grown at steady state under both reductiveand oxidative stress and the intracellular fluxes weredetermined by mass-balancing techniques. By decreasing the dissolved oxygen pressure (pO(2)) in the bioreactor, the reduced formof ni
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4b0f2d9be9f8738be1927a32b2ca8b53
https://pubmed.ncbi.nlm.nih.gov/19002972
https://pubmed.ncbi.nlm.nih.gov/19002972
Publikováno v:
Cytotechnology. 29(3)
The analysis of metabolic fluxes of large stoichiometric systems is sensitive to measurement errors in metabolic uptake and production rates. It is therefore desirable to independently test the consistency of measurement data, which is possible if at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::db5444baa7ac5e6874f0b1b21c6a705e
https://pubmed.ncbi.nlm.nih.gov/19003341
https://pubmed.ncbi.nlm.nih.gov/19003341
Publikováno v:
Bioprocess and Biosystems Engineering 26 (2003)
Bioprocess and Biosystems Engineering, 26, 123-132
Bioprocess and Biosystems Engineering, 26, 123-132
A model is presented that describes all the saccharides that are produced during the hydrolysis of starch by an alpha-amylase. Potato amylopectin, the substrate of the hydrolysis reaction, was modeled in a computer matrix. The four different subsite
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93805306df6cf80bf3eeae876bd8e023
https://pubmed.ncbi.nlm.nih.gov/14618387
https://pubmed.ncbi.nlm.nih.gov/14618387
Publikováno v:
Biotechnology and bioengineering. 58(2-3)
The estimation of intracellular fluxes of mammalian cells using only mass balances of the relevant metabolites is not possible because the set of linear equations defined by these mass balances is underdetermined. In order to quantify fluxes in cycli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::84b0fee6122a5e2d05c9211c4a2ff72c
https://pubmed.ncbi.nlm.nih.gov/10191398
https://pubmed.ncbi.nlm.nih.gov/10191398
Publikováno v:
Biotechnology and bioengineering. 57(4)
The effect of added ammonia on the intracellular fluxes in hybridoma cells was investigated by metabolic-flux balancing techniques. It was found that, in ammonia-stressed hybridoma cells, the glutamate-dehydrogenase flux is in the reverse direction c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::055c9d1ad3e75041ab46babe76d3e662
https://pubmed.ncbi.nlm.nih.gov/10099221
https://pubmed.ncbi.nlm.nih.gov/10099221
Publikováno v:
Biotechnology and bioengineering. 62(3)
The hydrolysis of amylopectin potato starch with Bacillus licheniformis alpha-amylase (Maxamyl) was studied under industrially relevant conditions (i.e. high dry-weight concentrations). The following ranges of process conditions were chosen and inves
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cb5545cf4343c6163d22acf771e98c64
https://pubmed.ncbi.nlm.nih.gov/10099546
https://pubmed.ncbi.nlm.nih.gov/10099546
Publikováno v:
Bioseparation. 7(2)
The effect of the important ATPS- and buffer parameters on IgG and hybridoma partitioning in ATPSs containing a PEG-dye-ligand was studied. Objective was to establish selection criteria for effective ligands for extractive fermentations with animal c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::57d569c6a3ebc54b14e9a8fe949908a7
https://pubmed.ncbi.nlm.nih.gov/9763700
https://pubmed.ncbi.nlm.nih.gov/9763700
Autor:
Bram Timmerarends, C. D. de Gooijer, Peter Skrabal, Elmar Heinzle, Georg Schmid, Johannes Tramper, Ahmet Ozemre, H.P.J. Bonarius
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
The analysis of the intracellular metabolic fluxes in mammalian cells can be used for optimizing culture media, for tracing metabolic bottle-necks and for toxicological and metabolic studies. It is desirable to determine metabolic fluxes without usin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3d6d5c3d48b6ded5c0b73156078f4d7f
https://doi.org/10.1007/978-94-011-5404-8_99
https://doi.org/10.1007/978-94-011-5404-8_99
