Single‐cell profiling screen identifies microtubule‐dependent reduction of variability in signaling

Autor: C Gustavo Pesce, Stefan Zdraljevic, William J Peria, Alan Bush, María Victoria Repetto, Daniel Rockwell, Richard C Yu, Alejandro Colman‐Lerner, Roger Brent

Publikováno v: Molecular Systems Biology, Vol 14, Iss 4, Pp 1-20 (2018)

Abstract Populations of isogenic cells often respond coherently to signals, despite differences in protein abundance and cell state. Previously, we uncovered processes in the Saccharomyces cerevisiae pheromone response system (PRS) that reduced cell�

Externí odkaz: https://doaj.org/article/395a3c70030f4b1eae69f1e78f2d5bde

Expression of the receptor activator of nuclear factor-kB ligand in peripheral blood mononuclear cells in patients with acute charcot neuroarthropathy

Autor: Maria Teresa Mondillo, Giada Veneziano, Luigi Uccioli, Laura De Corato, Alberto Bergamini, Valentina Girardi, Francesca Bolacchi, C. D. Pesce, Ettore Squillaci

Publikováno v: International Journal of Medical Sciences

Introduction. The receptor activator of nuclear factor-kB (RANK), ligand (RANK-L) and osteoprotegerin (OPG) are implicated in the pathogenesis of acute Charcot neuroarthropathy (CN). Materials and Methods. This study aimed to investigate the expressi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0af88bde8dfd7dad9a6087eccd1cb60b
http://hdl.handle.net/2108/171310

Negative feedback that improves information transmission in yeast signalling

Autor: C. Gustavo Pesce, Eduard Serra, Kirsten R. Benjamin, Mark R. Holl, Andrew S. Gordon, Larry Lok, Alejandro Colman-Lerner, David Pincus, Roger Brent, Richard C. Yu

Publikováno v: Nature. 456:755-761

Haploid Saccharomyces cerevisiae yeast cells use a prototypic cell signalling system to transmit information about the extracellular concentration of mating pheromone secreted by potential mating partners. The ability of cells to respond distinguisha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f2cdc6b0f5d8b990d3bfab32959f83ec
https://doi.org/10.1038/nature07513

Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae

Autor: Marilyn G. Wiebe, Mervi Toivari, Dominik Mojzita, C. Gustavo Pesce, Laura Ruohonen, Hannu Maaheimo, Yvonne Nygård, Orna Resnekov, Merja Penttilä

Publikováno v: Nygård, Y, Maaheimo, H, Mojzita, D, Toivari, M, Wiebe, M, Resnekov, O, Gustavo Pesce, C, Ruohonen, L & Penttilä, M 2014, ' Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae ', Metabolic Engineering, vol. 25, pp. 238-247 . https://doi.org/10.1016/j.ymben.2014.07.005

D-xylonate is a potential platform chemical which can be produced by engineered Saccharomyces cerevisiae strains. In order to address production constraints in more detail, we analysed the role of lactone ring opening in single cells and populations.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6aba655e8a10555df274744449c3e212
https://pubmed.ncbi.nlm.nih.gov/25073011

Functional association between promoter structure and transcript alternative splicing

Autor: Paula Cramer, Alberto R. Kornblihtt, Francisco E. Baralle, C G Pesce

Publikováno v: Proceedings of the National Academy of Sciences. 94:11456-11460

It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the RNA molecule. Here we show that changes in promoter structure strongly affect splice site selection. We invest

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27e6ff4dbed31228dd425f92121a0ad5
https://doi.org/10.1073/pnas.94.21.11456