A protocol for recombinant protein quantification by densitometry

Autor: Susana María Alonso Villela, Hazar Kraïem, Balkiss Bouhaouala‐Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau

Publikováno v: MicrobiologyOpen, Vol 9, Iss 6, Pp 1175-1182 (2020)

Abstract The protein purity is generally checked using SDS‐PAGE, where densitometry could be used to quantify the protein bands. In literature, few studies have been reported using image analysis for the quantification of protein in SDS‐PAGE: tha

Externí odkaz: https://doaj.org/article/edbb51fd353f4dc8b9d44c37fc28b4a8

Biochemical limitations of Bacillus thuringiensis based biopesticides production in a wheat bran culture medium

Autor: Rita Barssoum, Gabrielle Al Kassis, Rayan Nasseredine, Jihane Saad, Meriem El Ghoul, Joanna Abboud, Nancy Fayad, Stéphanie Dupoiron, Julien Cescut, César Arturo Aceves-Lara, Luc Fillaudeau, Mireille Kallassy Awad

Publikováno v: Research in Microbiology. :104043

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bc799465cc30a5be6359c2cfedc6cf37
https://doi.org/10.1016/j.resmic.2023.104043

Effect of temperature on the production of a recombinant antivenom in fed-batch mode

Autor: Balkiss Bouhaouala-Zahar, César Arturo Aceves Lara, Hazar Ghezal-Kraïem, Susana María Alonso Villela, Carine Bideaux, Luc Fillaudeau

Publikováno v: Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology, Springer Verlag, 2021, 105 (3), pp.1017-1030. ⟨10.1007/s00253-021-11093-5⟩
Applied Microbiology and Biotechnology, 2021, 105 (3), pp.1017-1030. ⟨10.1007/s00253-021-11093-5⟩

In the pharmaceutical industry, nanobodies show promising properties for its application in serotherapy targeting the highly diffusible scorpion toxins. The production of recombinant nanobodies in Escherichia coli has been widely studied in shake fla

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87282c42e18b9cbb54857eb637f63332
https://hal.inrae.fr/hal-03124955

2-Phenylethanol and 2-phenylethylacetate production by nonconventional yeasts using tequila vinasses as a substrate

Autor: César Arturo Aceves-Lara, Lorena Amaya-Delgado, José de Jesús Rodríguez-Romero, Anne Christine Gschaedler, Javier Arrizon, Cristina Ferreira Silva

Publikováno v: Biotechnology Reports, Vol 25, Iss, Pp-(2020)
Biotechnology Reports

Highlights • Yeast species influenced the de novo synthesis of 2-phenylethylacetate. • Inhibitory compounds showed a strong influence on cell growth and 2-phenylethylacetate production for the evaluated yeasts. • More than a 50 % reduction in t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39ed72f1d403e979f25fcd96caa8bd4d
https://doi.org/10.1016/j.btre.2020.e00420

Modeling and optimization of lipid accumulation by Yarrowia lipolytica from glucose under nitrogen depletion conditions

Autor: Nathalie Gorret, Rafael Muñoz-Tamayo, Carine Bideaux, César Arturo Aceves-Lara, C.E. Robles-Rodriguez, Carole Molina-Jouve, Gilles Roux, Stéphane E. Guillouet

Publikováno v: Biotechnology and Bioengineering
Biotechnology and Bioengineering, 2018, 115 (5), pp.1-15. ⟨10.1002/bit.26537⟩
Biotechnology and Bioengineering, Wiley, 2018, pp.1-15. ⟨10.1002/bit.26537⟩
Biotechnology and Bioengineering 5 (115), 1137-1151. (2018)
Biotechnology and Bioengineering, Wiley, 2018, 115 (5), pp.1-15. ⟨10.1002/bit.26537⟩

International audience; Oleaginous yeasts have been seen as a feasible alternative to produce the precursors of biodiesel due to their capacity to accumulate lipids as triacylglycerol having profiles with high content of unsaturated fatty acids. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23312efbdd01f1831d655b55b6776c63
https://hal.science/hal-01713779/file/2018_Robles_Biotechnol-Bioeng_1