Distinct dynamics of mRNA LNPs in mice and nonhuman primates revealed by in vivo imaging

Autor: Katia Lemdani, Romain Marlin, Céline Mayet, Vladimir Perkov, Quentin Pascal, Manon Ripoll, Francis Relouzat, Nina Dhooge, Laetitia Bossevot, Nathalie Dereuddre-Bosquet, Gihad Dargazanli, Kevin Thibaut-Duprey, Jean Haensler, Catherine Chapon, Christine Prost, Roger Le Grand

Publikováno v: npj Vaccines, Vol 9, Iss 1, Pp 1-9 (2024)

Abstract The characterization of vaccine distribution to relevant tissues after in vivo administration is critical to understanding their mechanisms of action. Vaccines based on mRNA lipid nanoparticles (LNPs) are now being widely considered against

Externí odkaz: https://doaj.org/article/9a16683cef5943cfbca28efe52bdabf2

Huntingtin proteolysis releases non‐polyQ fragments that cause toxicity through dynamin 1 dysregulation

Autor: Marie‐Thérèse El‐Daher, Sandrine Lévêque-Fort, Juan Botas, Ismael Al-Ramahi, Emilie Hangen, Ghislaine Poizat, Raul Pardo, Julie Bruyère, Sandrine Humbert, Nicolas Bourg, Sylvie Souquere, Gérard Pierron, Frédéric Saudou, Céline Mayet

Publikováno v: EMBO Journal
EMBO Journal, EMBO Press, 2015, 34 (17), pp.2255-2271. ⟨10.15252/embj.201490808⟩
EMBO Journal, 2015, 34 (17), pp.2255-2271. ⟨10.15252/embj.201490808⟩

International audience; Cleavage of mutant huntingtin (HTT) is an essential process in Huntington's disease (HD), an inherited neurodegenerative disorder. Cleavage generates N-ter fragments that contain the polyQ stretch and whose nuclear toxicity is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4a34309be7a95cc6aa051b31fa90b3d
https://hal.archives-ouvertes.fr/hal-03297282

Confocal supercritical angle microscopy for cell membrane imaging

Autor: Emmanuel Fort, Sandrine Lévêque-Fort, Céline Mayet, Thomas Barroca, Siddharth Sivankutty, Guillaume Dupuis

Publikováno v: Optics letters. 39(3)

We demonstrate subwavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fluorescence, wherein only a fluorophore next to the interface of a re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16e96b308b696da83adfa73fccdf10a9
https://pubmed.ncbi.nlm.nih.gov/24487864

Towards STED microscopy with nanometric optical sectioning (Orale)

Autor: Guillaume Dupuis, Sandrine Lecart, Arnaud Dubois, Christophe Lefumeux, Sandrine Lévêque-Fort, Céline Mayet, Marie-Claude Potier, Siddharth Sivankutty, Emmanuel Fort, Thomas Barroca, Catherine Marquer

Publikováno v: Proc. SPIE
Photonics West-Biomedical Optics, Single molecule spectroscopy and superresolution imaging VI
Photonics West-Biomedical Optics, Single molecule spectroscopy and superresolution imaging VI, Feb 2013, San Francisco, United States. pp.859018, ⟨10.1117/12.2004883⟩

International audience; Circumventing the limit imposed by diffraction is a major issue in the instrumental development to realize finer resolutions in biological samples. With STED microscopy, we exploit the molecular transitions of the fluorescent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dd86f2bda1f520b3fd684c7ebd67859
https://hal-iogs.archives-ouvertes.fr/hal-00835893

Sub-100 nm IR spectromicroscopy of living cells

Autor: Jean-Michel Ortega, F. Glotin, Alexandre Dazzi, Rui Prazeres, F. Allot, Céline Mayet

Publikováno v: Optics letters. 33(14)

We have performed IR spectromicroscopy of cells immersed in liquid water, with a lateral resolution better than 100 nm. Here, we use the motion of an atomic force microscope tip, probing the local transient deformation induced by an IR pulsed laser t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac20cedf0455cc4d83e3738198f2716a
https://pubmed.ncbi.nlm.nih.gov/18628814