Comparison of DNA extraction methods for 16S rRNA gene sequencing in the analysis of the human gut microbiome

Autor: Céline Elie, Magali Perret, Hayat Hage, Erwin Sentausa, Amy Hesketh, Karen Louis, Asmaà Fritah-Lafont, Philippe Leissner, Carole Vachon, Hervé Rostaing, Frédéric Reynier, Gaspard Gervasi, Adrien Saliou

Publikováno v: Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)

Abstract The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S rRNA gene amplicon sequencing and shotgun metagenomic sequencing (SMS). DNA extraction is known to have a large impact on the metagenomic analyses. The aim o

Externí odkaz: https://doaj.org/article/7826261ebfde457c834fb1d876f20798

A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes

Autor: Marion Darnaud, Filipe De Vadder, Pascaline Bogeat, Lilia Boucinha, Anne-Laure Bulteau, Andrei Bunescu, Céline Couturier, Ana Delgado, Hélène Dugua, Céline Elie, Alban Mathieu, Tereza Novotná, Djomangan Adama Ouattara, Séverine Planel, Adrien Saliou, Dagmar Šrůtková, Jennifer Yansouni, Bärbel Stecher, Martin Schwarzer, François Leulier, Andrea Tamellini

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-21 (2021)

Here, the authors develop and characterize a mouse microbiota composed of 15 strains representative of the intestinal microbiota found in C57BL/6J specific opportunistic- and pathogen-free (C57Bl/6J SOPF) mice and derive a new standardized gnotobioti

Externí odkaz: https://doaj.org/article/ad2f54703d4e4a40b40755a7d77374ec

Regenerable ZnO/GaAs Bulk Acoustic Wave Biosensor for Detection of Escherichia coli in 'Complex' Biological Medium

Autor: Juliana Chawich, Walid M. Hassen, Céline Elie-Caille, Thérèse Leblois, Jan J. Dubowski

Publikováno v: Biosensors, Vol 11, Iss 5, p 145 (2021)

A regenerable bulk acoustic wave (BAW) biosensor is developed for the rapid, label-free and selective detection of Escherichia coli in liquid media. The geometry of the biosensor consists of a GaAs membrane coated with a thin film of piezoelectric Zn

Externí odkaz: https://doaj.org/article/d2f71373bc744bc4bdbba80a65ce7408

Implementation of capacitance as simultaneous sensing and actuating tool in tapered microelectrode arrays for dielectrophoresis-on-a-chip application

Autor: Buyong, Muhamad Ramdzan, Larki, Farhad, Hakimi Zainal, Muhamad Ikhmal, Almahi, Abdelaziz Yousif Ahmed, Ismail, Ahmad Ghadafi, Hamzah, Azrul Azlan, Kayani, Aminuddin Ahmad Kayani, Caille, Céline Elie, Yeop Majlis, Burhanuddin

Publikováno v: Microelectronics International, 2020, Vol. 37, Issue 4, pp. 215-224.

Externí odkaz: http://www.emeraldinsight.com/doi/10.1108/MI-04-2020-0023

Development of extracellular vesicle-based medicinal products: A position paper of the group 'Extracellular Vesicle translatiOn to clinicaL perspectiVEs – EVOLVE France'

Autor: Emilie Velot, Marina Trouillas, Joël Chopineau, J. Peltzer, Sophie Brouard, Olivier Favre-Bulle, Jérôme Larghero, Tristan Montier, Amanda K. A. Silva, Florence Gazeau, Phlippe Mauduit, Jean-Hugues Trouvin, Arnaud Bianchi, Noëlle Mathieu, Christian Jorgensen, Matti Ullah, Kelly Aubertin, Philippe Menasché, Surendar Arumugam, Jolanda Spadavecchia, C. Aussel, Danièle Noël, Wilfrid Boireau, M. Dedier, Sébastien Jauliac, Sébastien Banzet, Jean-Marie Bach, Antoine Monsel, Max Piffoux, Kondareddy Cherukula, Marie Morille, Célia Ravel, Christophe Martinaud, Chantal M. Boulanger, Claire Wilhelm, Mathilde Mosser, Olivier Blanc-Brude, Nicolas Sailliet, Pierre-Emmanuel Rautou, Nathalie Luciani, Isabelle Raymond-Letron, Céline Elie-Caille, Gabriel Rahmi, Anna C. Sebbagh

Publikováno v: Advanced Drug Delivery Reviews
Advanced Drug Delivery Reviews, Elsevier, 2021, 179, pp.114001. ⟨10.1016/j.addr.2021.114001⟩
Advanced Drug Delivery Reviews, 2021, 179, pp.114001. ⟨10.1016/j.addr.2021.114001⟩

International audience; Extracellular vesicles (EV) are emergent therapeutic effectors that have reached clinical trial investigation. To translate EV-based therapeutic to clinic, the challenge is to demonstrate quality, safety, and efficacy, as requ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::607492892c07dcbf68c27bb32eea9b55
https://hal.archives-ouvertes.fr/hal-03412034