Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Bushra Raj"'
Publikováno v:
Nature Protocols. 13:2685-2713
Lineage relationships among the large number of heterogeneous cell types generated during development are difficult to reconstruct in a high-throughput manner. We recently established a method, scGESTALT, that combines cumulative editing of a lineage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f392a1627ed4d08d1865c8e8e57a1c41
https://doi.org/10.1038/s41596-018-0058-x
https://doi.org/10.1038/s41596-018-0058-x
Autor:
Aaron McKenna, Alexander F. Schier, James A. Gagnon, Jay Shendure, Allon M. Klein, Bushra Raj, Shristi Pandey, Daniel E. Wagner
Publikováno v:
Nature Biotechnology. 36:442-450
The lineage relationships among the hundreds of cell types generated during development are difficult to reconstruct. A recent method, GESTALT, used CRISPR-Cas9 barcode editing for large-scale lineage tracing, but was restricted to early development
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::52728e8710c13f3755da3bd541167a39
https://doi.org/10.1038/nbt.4103
https://doi.org/10.1038/nbt.4103
Neurogenesis in the vertebrate brain comprises many steps ranging from the proliferation of progenitors to the differentiation and maturation of neurons. Although these processes are highly regulated, the landscape of transcriptional changes and prog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb9598592f4a64c397a2f96c719affca
Autor:
Allon M. Klein, Daniel E. Wagner, Jay Shendure, Bushra Raj, James A. Gagnon, Alexander F. Schier, Aaron McKenna, Shristi Pandey
Hundreds of cell types are generated during development, but their lineage relationships are largely elusive. Here we report a technology, scGESTALT, which combines cell type identification by single-cell RNA sequencing with lineage recording by cumu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efe464fee6871b9fb79001c12f690480
https://doi.org/10.1101/205534
https://doi.org/10.1101/205534
Autor:
Jernej Ule, Benjamin J. Blencowe, Anne-Claude Gingras, Zhen-Yuan Lin, Laura E. Easton, Bushra Raj, Manuel Irimia, Timothy Sterne-Weiler, Ginny I. Chen, Dave O'Hanlon, Ulrich Braunschweig, Eduardo Eyras
Publikováno v:
Molecular Cell. 56:90-103
The vertebrate and neural-specific Ser/Arg (SR)-related protein nSR100/SRRM4 regulates an extensive program of alternative splicing with critical roles in nervous system development. However, the mechanism by which nSR100 controls its target exons is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76d810fd8ef1503b31e3583bcaa2c3da
https://doi.org/10.1016/j.molcel.2014.08.011
https://doi.org/10.1016/j.molcel.2014.08.011
Autor:
Qun Pan, Debashish Ray, Freda D. Miller, Dave O'Hanlon, Bushra Raj, John P. Vessey, Noel J. Buckley, Benjamin J. Blencowe
Publikováno v:
Molecular Cell. 43(5):843-850
Neurogenesis requires the concerted action of numerous genes that are regulated at multiple levels. However, how different layers of gene regulation are coordinated to promote neurogenesis is not well understood. We show that the neural-specific Ser/
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5861f8c3fccb6fbc07ff34b0877222b8
Autor:
Jonathan Roth, Benjamin J. Blencowe, Jonathan D. Ellis, Jason Moffat, Bushra Raj, Anne-Claude Gingras, Thomas Gonatopoulos-Pournatzis, Mingkun Wu, Dave O'Hanlon, John A. Calarco, Hong Han, Andrew J. Best, Ulrich Braunschweig, Michael Aregger
Publikováno v:
Molecular Cell. 72:510-524.e12
Alternative splicing is crucial for diverse cellular, developmental, and pathological processes. However, the full networks of factors that control individual splicing events are not known. Here, we describe a CRISPR-based strategy for the genome-wid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6db4ab82468ba8dce08ca350693f415a
https://doi.org/10.1016/j.molcel.2018.10.008
https://doi.org/10.1016/j.molcel.2018.10.008
Autor:
Anne-Claude Gingras, Bushra Raj, Thomas Gonatopoulos-Pournatzis, Benjamin J. Blencowe, Zhen-Yuan Lin, Serge Gueroussov, Manuel Irimia
Alternative splicing (AS) generates extensive transcriptomic and proteomic complexity. However, the functions of species- and lineage-specific splice variants are largely unknown. Here we show that mammalian-specific skipping of polypyrimidine tract-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84bf807efb802a935f2ae2587f4e247e
http://hdl.handle.net/10230/27120
http://hdl.handle.net/10230/27120
Autor:
Qun Pan, Simone Superina, Mathieu Gabut, Danielle Gelinas, Laura Clarke, John A. Calarco, Ursula Skalska, Benjamin J. Blencowe, Bushra Raj, Derek van der Kooy, Brian Ciruna, Dave O'Hanlon, Mei Zhen
Publikováno v:
Cell. (5):898-910
SummaryAlternative splicing is a key process underlying the evolution of increased proteomic and functional complexity and is especially prevalent in the mammalian nervous system. However, the factors and mechanisms governing nervous system-specific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08a53a614641df5556eec64b5d75a342
Autor:
Benjamin J. Blencowe, Bushra Raj
Publikováno v:
Neuron. (1):14-27
High-throughput transcriptomic profiling approaches have revealed that alternative splicing (AS) of precursor mRNAs, a fundamental process by which cells expand their transcriptomic diversity, is particularly widespread in the nervous system. AS even
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18cbb017095c3d4bbf92d11bfd26f5af