Terminally truncated isopenicillin N synthase generates a dithioester product: evidence for a thioaldehyde intermediate during catalysis and a new mode of reaction for non-heme iron oxidases

Autor: McNeill, Luke A., Brown, Toby J. N., Sami, Malkit, Clifton, Ian J., Burzlaff, Nicolai I., Claridge, Timothy D. W., Adlington, Robert M., Baldwin, Jack E., Rutledge, Peter J., Schofield, Christopher J.

Publikováno v: 'Chemistry-A European Journal ', vol: 23, pages: 12815-12824 (2017)
Chemistry (Weinheim an Der Bergstrasse, Germany)

Isopenicillin N synthase (IPNS) catalyses the four‐electron oxidation of a tripeptide, l‐δ‐(α‐aminoadipoyl)‐l‐cysteinyl‐d‐valine (ACV), to give isopenicillin N (IPN), the first‐formed β‐lactam in penicillin and cephalosporin bi

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X-ray Crystal Structure of Escherichia coli Taurine/α-Ketoglutarate Dioxygenase Complexed to Ferrous Iron and Substrates.

Autor: Elkins, Jonathan M., Ryle, Matthew J., Clifton, Ian J., Hotopp, Julie C. Dunning, Lloyd, John S., Burzlaff, Nicolai I., Baldwin, Jack E., Hausinger, Robert P., Roach, Peter L.

Publikováno v: Biochemistry; 4/23/2002, Vol. 41 Issue 16, p5185, 8p, 4 Color Photographs, 3 Diagrams, 2 Charts

Terminally Truncated Isopenicillin N Synthase Generates a Dithioester Product: Evidence for a Thioaldehyde Intermediate during Catalysis and a New Mode of Reaction for Non-Heme Iron Oxidases.

Autor: McNeill LA; Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK.; Present Address: Oxford Nanopore Technologies, Oxford Science Park, OX4 4GA, UK., Brown TJN; Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK.; Present Address: The Brattle Group, Level 15 5 Martin Place, Sydney, NSW, 2000, Australia., Sami M; Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK.; Present Address: Immunocore Limited, 101 Park Drive, Milton Park, Abingdon, OX14 4RY, UK., Clifton IJ; Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK., Burzlaff NI; Department of Chemistry and Pharmacy, University of Erlangen-Nuremberg, Egerlandstraße 1, 91058, Erlangen, Germany., Claridge TDW; Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK., Adlington RM; Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK., Baldwin JE; Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK., Rutledge PJ; School of Chemistry, The University of Sydney, NSW, 2006, Australia., Schofield CJ; Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK.

Publikováno v: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2017 Sep 18; Vol. 23 (52), pp. 12815-12824. Date of Electronic Publication: 2017 Aug 21.