Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Burkhard, Wilms"'
Surface display vectors for selective detection and isolation of high level antibody producing cells
Autor:
Hans-Peter Knopf, Thomas Jostock, Sabine Lang, Johannes Wichter, Nommay Audrey Josiane, Delia Drewello, Burkhard Wilms
Publikováno v:
Biotechnology and Bioengineering. 113:2386-2393
Cell line generation for production of biopharmaceuticals in mammalian cells usually involves intensive screening of clones to identify the rare high producers. In order to facilitate efficient and selective fluorescence activated cell sorting (FACS)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bf13b9ad2f5bf360a45f597743d08ae3
https://doi.org/10.1002/bit.26000
https://doi.org/10.1002/bit.26000
Autor:
Holger Laux, Thomas Jostock, Anett Ritter, Joerg Schmidt, Burkhard Wilms, Mara Fornaro, Sandrine Romand
Publikováno v:
Biotechnology and Bioengineering. 113:1094-1101
Chinese Hamster Ovary (CHO) cells are widely used for the large-scale production of recombinant biopharmaceuticals. However, attempts to express IGF-1 (a mutated human Insulin-like growth factor 1 Ea peptide (hIGF-1Ea mut)) in CHO cells resulted in p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::493d6070e2642b1402f4877569c0c4ed
https://doi.org/10.1002/bit.25877
https://doi.org/10.1002/bit.25877
Autor:
Anett Ritter, Thomas Jostock, Sabine Geisse, Burkhard Wilms, Holger Laux, Johannes Wienberg, Bernd Voedisch
Publikováno v:
Biotechnology and Bioengineering. 113:1084-1093
Chinese Hamster Ovary (CHO) cells are widely used for large scale production of recombinant biopharmaceuticals. Although these cells have been extensively used, a demand to further increase the performance, for example, to facilitate the process of c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1375bc89fc530afb6bc1a36935856e45
https://doi.org/10.1002/bit.25876
https://doi.org/10.1002/bit.25876
Surface display vectors for selective detection and isolation of high level antibody producing cells
Autor:
Sabine, Lang, Delia, Drewello, Johannes, Wichter, Audrey, Nommay, Burkhard, Wilms, Hans-Peter, Knopf, Thomas, Jostock
Publikováno v:
Biotechnology and bioengineering. 113(11)
Cell line generation for production of biopharmaceuticals in mammalian cells usually involves intensive screening of clones to identify the rare high producers. In order to facilitate efficient and selective fluorescence activated cell sorting (FACS)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::732a28ca3a53addb69912018b840ae45
https://pubmed.ncbi.nlm.nih.gov/27144878
https://pubmed.ncbi.nlm.nih.gov/27144878
Publikováno v:
BMC Proceedings
Background Chinese hamster ovary (CHO) cells are the most widely used host for large scale production of recombinant therapeutic proteins. A combination of several gene editing approaches applied to Novartis proprietary CHO cell line resulted in a su
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::435c7747880021c4b1c265031b1a31b3
https://doi.org/10.1186/1753-6561-9-s9-p17
https://doi.org/10.1186/1753-6561-9-s9-p17
Autor:
Anett, Ritter, Bernd, Voedisch, Johannes, Wienberg, Burkhard, Wilms, Sabine, Geisse, Thomas, Jostock, Holger, Laux
Publikováno v:
Biotechnology and bioengineering. 113(5)
Chinese Hamster Ovary (CHO) cells are widely used for large scale production of recombinant biopharmaceuticals. Although these cells have been extensively used, a demand to further increase the performance, for example, to facilitate the process of c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2f9bb1d0a8e9c3248cc14c0e533482a4
https://pubmed.ncbi.nlm.nih.gov/26523402
https://pubmed.ncbi.nlm.nih.gov/26523402
Autor:
Sandrine, Romand, Thomas, Jostock, Mara, Fornaro, Joerg, Schmidt, Anett, Ritter, Burkhard, Wilms, Holger, Laux
Publikováno v:
Biotechnology and bioengineering. 113(5)
Chinese Hamster Ovary (CHO) cells are widely used for the large-scale production of recombinant biopharmaceuticals. However, attempts to express IGF-1 (a mutated human Insulin-like growth factor 1 Ea peptide (hIGF-1Ea mut)) in CHO cells resulted in p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b31f06f65aba146c7dd3e906b5dca880
https://pubmed.ncbi.nlm.nih.gov/26523469
https://pubmed.ncbi.nlm.nih.gov/26523469
Publikováno v:
Applied Microbiology and Biotechnology. 55:750-757
The genes encoding hydantoinases (hyuH1) and carbamoylases (hyuC1) from Arthrobacter aurescens DSM 3745 and Arthrobacter aurescens DSM 3747 (hyuH2, hyuC2) were cloned in Escherichia coli and the nucleotide sequences determined. The hydantoinase genes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a8563eee86a8386bd46643635f566ec
https://doi.org/10.1007/s002530000574
https://doi.org/10.1007/s002530000574
Autor:
Matthias Reuss, Burkhard Wilms, Martin Siemann, Ralf Mattes, Josef Altenbuchner, Achim Hauck, Christoph Syldatk
Publikováno v:
Biotechnology and Bioengineering. 73:95-103
A high-cell-density fed-batch fermentation for the production of heterologous proteins in Escherichia coli was developed using the positively regulated Escherichia coli rhaBAD promoter. The expression system was improved by reducing of the amount of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb01b82f07a8358bff5ee202f9f4fca8
https://doi.org/10.1002/bit.1041
https://doi.org/10.1002/bit.1041
Autor:
Anja Wiese, Christoph Syldatk, Josef Altenbuchner, Ralf Mattes, Markus Pietzsch, Burkhard Wilms
Publikováno v:
Journal of Biotechnology. 68:101-113
An l -N-carbamoyl amino acid amidohydrolase ( l -N-carbamoylase) from Arthrobacter aurescens DSM 3747 was cloned in E. coli and the nucleotide sequence was determined. After expression of the gene in E. coli the enzyme was purified to homogeneity and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8464f84193272e342e08cbb429b7c50c
https://doi.org/10.1016/s0168-1656(98)00183-7
https://doi.org/10.1016/s0168-1656(98)00183-7