Tailoring FPOX enzymes for enhanced stability and expanded substrate recognition.

Autor: Estiri H; Department of Biotechnology, Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, 1006, Latvia., Bhattacharya S; Department of Biotechnology, Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, 1006, Latvia.; Faculty of Materials Science and Applied Chemistry, Riga Technical University, Paula Valdena 3, Riga, 1048, Latvia., Buitrago JAR; Department of Biotechnology, Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, 1006, Latvia., Castagna R; Department of Biotechnology, Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, 1006, Latvia.; Dipartimento di Chimica, Materiali e Ingegneria Chimica 'Giulio Natta', Politecnico di Milano, Piazza L. da Vinci 32, 20133, Milano, Italy., Legzdiņa L; Department of Biotechnology, Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, 1006, Latvia., Casucci G; Department of Biotechnology, Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, 1006, Latvia., Ricci A; Biomolecular Engineering Lab, Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133, Milano, Italy., Parisini E; Department of Biotechnology, Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, 1006, Latvia. emilio.parisini@osi.lv.; Department of Chemistry 'G. Ciamician', University of Bologna, Via Selmi 2, 40126, Bologna, Italy. emilio.parisini@osi.lv., Gautieri A; Biomolecular Engineering Lab, Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133, Milano, Italy. alfonso.gautieri@polimi.it.

Publikováno v: Scientific reports [Sci Rep] 2023 Oct 30; Vol. 13 (1), pp. 18610. Date of Electronic Publication: 2023 Oct 30.

CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering*.

Autor: Bracco P; Biocatalysis, Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany., Wijma HJ; Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, Netherlands., Nicolai B; Biocatalysis, Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany., Buitrago JAR; Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany., Klünemann T; Structure and Function of Proteins, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany., Vila A; Biocatalysis, Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany., Schrepfer P; Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany., Blankenfeldt W; Structure and Function of Proteins, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.; Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany., Janssen DB; Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, Netherlands., Schallmey A; Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.

Publikováno v: Chembiochem : a European journal of chemical biology [Chembiochem] 2021 Mar 16; Vol. 22 (6), pp. 1099-1110. Date of Electronic Publication: 2020 Nov 30.

Comparative stability of ficin and papain in acidic conditions and the presence of ethanol.

Autor: Milošević, Jelica, Janković, Brankica, Prodanović, Radivoje, Polović, Natalija

Publikováno v: Amino Acids; May2019, Vol. 51 Issue 5, p829-838, 10p