Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Bryan Dunbar"'
Publikováno v:
European Journal of Biochemistry. 260:250-257
Plant mitochondria from both potato and mung bean incorporated radioactivity into acid insoluble material when incubated with labelled polyamines (spermine, spermidine and putrescine). Extensive washing of mitochondrial precipitates with trichloroace
Publikováno v:
Plant Molecular Biology Reporter. 15:46-61
Identification of the 329 spots visible in 2D gels of plant mitochondrial proteins is a challenge. This paper describes a 2D mini-gel protocol involving free-radical scavengers and purified reagents to make it compatible with protein sequencing, and
Autor:
Carolyn A. Converse, John V. Forrester, John E. Fothergill, James L. Thomson, Henry Brzeski, Bryan Dunbar
Publikováno v:
Current Eye Research. 16:741-745
PURPOSE. To isolate and sequence cDNA for bovine rim protein, a large membrane-bound glycoprotein found in photoreceptor outer segments. METHODS. Bovine rim protein was N-terminally sequenced (22 residues) and fragments were prepared by partial prote
Publikováno v:
Microbiology. 141:1377-1383
Catalase was purified from the Gram-positive bacterium Streptomyces coelicolor A3(2) in a three-step purification procedure comprising (NH4)2SO4, fractionation, Phenyl-Sepharose chromatography and Mono Q chromatography. The purification of catalase,
Autor:
Richard J. Weaver, John E. Fothergill, William T. Melvin, M. Danny Burke, Bryan Dunbar, Maurice Dickins
Publikováno v:
Biochemical Pharmacology. 47:1457-1460
Evidence is presented for a new 3-methylcholanthrene (3MC)-induced form of cytochrome P450, P450MCX, in rat liver microsomes. P450MCX was co-purified with CYP1A1 from 3MC-treated male Sprague-Dawley rats but was resolved by gel electrophoresis. The M
Autor:
J E Fothergill, Nicholas C. Price, Jacqueline Nairn, Linda A. Fothergill-Gilmore, Graeme Walker, Bryan Dunbar
Publikováno v:
Biochemical Journal. 297:603-608
The amino acid sequence of the monomeric 2,3-bisphosphoglycerate (BPG)-dependent phosphoglycerate mutase (PGAM) from the fission yeast Schizosaccharomyces pombe has been determined. Amino acid sequencing of proteolytic fragments of the enzyme showed
Autor:
Julian Overnell, Peter Hunziker, Elaine A. Mackay, Bryan Dunbar, Ian Davidson, John E. Fothergill, Jeremias H. R. Kägi
Publikováno v:
European Journal of Biochemistry. 218:183-194
Cadmium-induced metallothioneins from the common sea mussel, Mytilus edulis, were shown to comprise of two groups of isoforms having apparent molecular masses of 10 kDa and 20 kDa. The 10-kDa group was resolved by anion-exchange chromatography into f
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1169:73-79
Fatty acid-binding proteins (FABPs) have been identified and purified from interscapular brown adipose tissue of the rat. The proteins were characterized and their properties compared with the FABP present in white adipose tissue. FABP was purified t
Autor:
Ruth M. Mould, Bryan Dunbar, Peter Weisbeek, Diane C. Bassham, Reinhold G. Herrmann, Dieter Bartling, Colin Robinson
Publikováno v:
Journal of Biological Chemistry. 266:23606-23610
The targeting of cytosolically synthesized proteins into the thylakoid lumen is mediated by an aminoterminal pre-sequence consisting of an "envelope transit" and a "thylakoid transfer" signal in tandem. We have investigated the structural characteris
Publikováno v:
European Journal of Biochemistry. 197:75-80
Penicillin acylase (EC 3.5.1.11) was completely inactivated with equimolar phenylmethane [35S]sulphonyl fluoride (PhMe35SO2F); the stability of the sulphonyl group in the modified protein was determined by measurement of the radioactivity in ultrafil