Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Bryan C Tieman"'
Autor:
Bryan C Tieman, Stephen Kovacs, Mary A Rodgers, Aurash Mohaimani, Svetoslava Gregory, David Christensen, Jeffrey A Moore, Lici Schurig-Briccio, James Hartnett, Alak Kar, Aaron Leeman, Angel Palmer, Lauren Rogers, Brian Dragoo, Steven Muszynski, Deborah Noblesmith, Samantha Gardner, Abigail Snipe-Bushey, Anna Kill, Xinxin Luo, Sneha Cherukuri, Tracey Rae, Christopher C Marohnic, Gavin A Cloherty, Anthony Scott Muerhoff, Philip M Hemken
Publikováno v:
BioTechniques, Vol 73, Iss 3, Pp 136-141 (2022)
Mutations in the nucleocapsid of SARS-CoV-2 may interfere with antigen detection by diagnostic tests. We used several methods to evaluate the effect of various SARS-CoV-2 nucleocapsid mutations on the performance of the Panbio™ and BinaxNOW™ late
Externí odkaz:
https://doaj.org/article/a1a5c21d6cb1492c89bd3b4de84b97b2
Autor:
Philip M. Hemken, Eitan Israeli, Russell Taylor, Chris Jacobson, Maria Datwyler, Rene Geissler, Abbas Hadji, Nicolette Jeanblanc, Kinnari Pandya, Marilee Marcinkus, Ryan Piktel, M. Felicia Bogdan, Mary Rodgers, Mark Anderson, Robert Ziemann, Bryan C. Tieman, David Hawksworth, Jeffrey Moore, Kathy S. Otis, Christopher C. Marohnic, Josie Corby, Bailin Tu, Zhihong Lin, Alak Kar, James Hartnett, Carolyn Strobel, Svetoslava Gregory, Tracey Rae, A. Scott Muerhoff, Susan Brophy, John R. Hackett, David Daghfal, Matthew L. Faron, Amorina Cruz, Phaedre Mohr, Lori Sokoll, Gerard J. Davis
Publikováno v:
Clinical Chemistry and Laboratory Medicine (CCLM).
Objectives To evaluate pre-analytical challenges related to high-volume central laboratory SARS-CoV-2 antigen testing with a prototype qualitative SARS-CoV-2 antigen immunoassay run on the automated Abbott ARCHITECT instrument. Methods Contrived posi
Autor:
Eitan, Israeli, Hideaki, Okura, Barry, Kreutz, Ryan, Piktel, Abbas, Hadji, Bailin, Tu, Zhihong, Lin, David J, Hawksworth, Bryan C, Tieman, Carolyn J, Strobel, Robert, Ziemann, Thomas P, Leary, A Scott, Muerhoff, Philip M, Hemken
Publikováno v:
Journal of Immunological Methods. 504:113262
Quantitative detection of interleukin-6 (IL-6) in serum and plasma can help monitor immune responses and the development of acute inflammation to guide patient management. We developed an IL-6 immunoassay for use with the automated ARCHITECT system f
Publikováno v:
Monoclonal antibodies in immunodiagnosis and immunotherapy. 36(3)
Monoclonal antibodies are widely used as the capture and detection reagents in diagnostic immunoassays. In the past, myeloma fusion partners expressing endogenous heavy and/or light chains were often used to generate hybridoma cell lines. As a result
Autor:
Joan D. Tyner, David J. Hawksworth, Bailin Tu, Bryan C. Tieman, A. Scott Muerhoff, Susan E. Brophy, Robert Ziemann
Publikováno v:
Hybridoma. 31:436-442
Development of a robust immunoassay requires the selection of monoclonal antibodies with desired properties. These properties generally include kinetics parameters such as on-rate and off-rate (i.e., binding affinity), and, often times, the ability t
Autor:
David J. Hawksworth, Susan E. Brophy, Bryan C. Tieman, Mary S. Pinkus, James W. Scheffel, Bailin Tu, Jeffrey M. Werneke, Joan D. Tyner, Robin A. Gutierrez, Robert Ziemann, Michael K. White
Publikováno v:
Clinical and Vaccine Immunology. 17:1040-1047
Mouse-human chimeric antibodies (cAbs) against hepatitis C virus (HCV) core, NS3 (nonstructural), NS4, and NS5 antigens were developed as quality control (QC) reagents to replace the use of human sera/plasma for Abbott HCV immunoassays. The cAb retai
Publikováno v:
Journal of Biological Chemistry. 276:44541-44550
Escherichia coli malate dehydrogenase (EcMDH) and its eukaryotic counterpart, porcine mitochondrial malate dehydrogenase (PmMDH), are highly homologous proteins with significant sequence identity (60%) and virtually identical native structural folds.
Autor:
Anthony M. Giannetti, Michael Vaughn, Klaus M. Michelson, Huawei Qiu, Jonathan Brooks, Giuseppe A. Papalia, Thomas E. Ryan, Anh Pham-Baginski, Michael M. Baksh, Katarzyna Matusiewicz, Rosemarie Wilton, Lakshman Bindu, Phinikoula S. Katsamba, Mieke Steukers, Yun Hee Cho, Johan Vandersmissen, Barry D. Bruce, Sanjay Phogat, Victoria A. Pudlak, Bryan C. Tieman, Siming Wang, David G. Myszka, Maggie A. Bynum, Lieve Emma Jan Michiels, Alexandra Smets, Stephanie Leavitt, Mieke De Wit, Agnes Nowakowski
Publikováno v:
Analytical biochemistry. 359(1)
In this benchmark study, 26 investigators were asked to characterize the kinetics and affinities of 10 sulfonamide inhibitors binding to the enzyme carbonic anhydrase II using Biacore optical biosensors. A majority of the participants collected data