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Akademický článek

'Usage a CRISPR/Cas9-based for obtaining knockouts of economically significant cattle genes'

Autor: Bruter A., Okulova Yu., Lysikova E., Silaeva Yu., Makutina V., Krivonogova A.

Publikováno v: E3S Web of Conferences, Vol 395, p 03008 (2023)

An experimental work dealing with the gene modification using the Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at the zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into th

Externí odkaz: https://doaj.org/article/bbdf2a3b077e46eca83eba7d9b8199ce

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Akademický článek

Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout

Autor: Silaeva Yulia Yu., Kubekina Marina V., Bruter Alexandra V., Isaeva Albina G., Koshchaev Andrey G.

Publikováno v: E3S Web of Conferences, Vol 176, p 01006 (2020)

Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for th

Externí odkaz: https://doaj.org/article/26623a801e4a47b098b94a4ae0ad3225

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Akademický článek

Production of hornless dairy cattle from genome-edited blastocysts

Autor: Deykin Alexey V., Bruter Alexandra V., Krivonogova Anna S., Shepelev Mikhail V., Koshchaev Andrey G.

Publikováno v: E3S Web of Conferences, Vol 176, p 01008 (2020)

Cattle of polled phenotype is convenient for breeders, as it decreases the risk of animals being hurt and ensures safety of workers. We developed the system for editing cattle genome using CRISPR/Cas9 which will allow production of animals with polle

Externí odkaz: https://doaj.org/article/49e4dabb14af47afbffbd729d430650e

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