Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag.

Autor: Brumbaugh-Reed EH; Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, USA.; Omenn-Darling Bioengineering Institute, Princeton University, Princeton, NJ, 08544, USA.; International Research Collaboration Center (IRCC), National Institutes of Natural Sciences, Tokyo, 105-0001, Japan., Gao Y; Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, USA., Aoki K; International Research Collaboration Center (IRCC), National Institutes of Natural Sciences, Tokyo, 105-0001, Japan.; Quantitative Biology Research Group, Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan.; Division of Quantitative Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan.; Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Aichi, 444-8787, Japan.; Laboratory of Cell Cycle Regulation Graduate School of Biostudies, Kyoto University, Kyoto, Kyoto, 606-8315, Japan., Toettcher JE; Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, USA. toettcher@princeton.edu.; Omenn-Darling Bioengineering Institute, Princeton University, Princeton, NJ, 08544, USA. toettcher@princeton.edu.

Publikováno v: Nature communications [Nat Commun] 2024 Aug 07; Vol. 15 (1), pp. 6717. Date of Electronic Publication: 2024 Aug 07.

pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells.

Autor: Farahani PE; Department of Chemical & Biological Engineering, Princeton University, Princeton, United States., Yang X; Department of Bioengineering, Rice University, Houston, United States.; Program in Systems, Synthetic, and Physical Biology, Rice University, Houston, United States., Mesev EV; Department of Molecular Biology, Princeton University, Princeton, United States., Fomby KA; Department of Molecular Biology, Princeton University, Princeton, United States., Brumbaugh-Reed EH; Department of Molecular Biology, Princeton University, Princeton, United States.; IRCC International Research Collaboration Center, National Institutes of Natural Sciences, Tokyo, Japan., Bashor CJ; Department of Bioengineering, Rice University, Houston, United States.; Department of Biosciences, Rice University, Houston, United States., Nelson CM; Department of Chemical & Biological Engineering, Princeton University, Princeton, United States.; Department of Molecular Biology, Princeton University, Princeton, United States., Toettcher JE; Department of Molecular Biology, Princeton University, Princeton, United States.

Publikováno v: ELife [Elife] 2023 May 22; Vol. 12. Date of Electronic Publication: 2023 May 22.