Role of nucleotide identity in effective CRISPR target escape mutations.

Autor: Künne T; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands.; Laboratory of Food Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands., Zhu Y; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands., da Silva F; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands., Konstantinides N; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands., McKenzie RE; Kavli Institute of Nanoscience, Department of Bionanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands., Jackson RN; Department of Chemistry and Biochemistry, Utah State University, 0300 Old Main Hill, Logan, UT, USA., Brouns SJ; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands.; Kavli Institute of Nanoscience, Department of Bionanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2018 Nov 02; Vol. 46 (19), pp. 10395-10404.

Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR-Cas system.

Autor: Staals RH; Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand., Jackson SA; Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand., Biswas A; Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand., Brouns SJ; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands., Brown CM; Department of Biochemistry, University of Otago, PO Box 56, Dunedin 9054, New Zealand.; Genetics Otago, University of Otago, PO Box 56, Dunedin 9054, New Zealand., Fineran PC; Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand.; Genetics Otago, University of Otago, PO Box 56, Dunedin 9054, New Zealand.; Bio-Protection Research Centre, University of Otago, PO Box 56, Dunedin 9054, New Zealand.

Publikováno v: Nature communications [Nat Commun] 2016 Oct 03; Vol. 7, pp. 12853. Date of Electronic Publication: 2016 Oct 03.

CRISPR interference and priming varies with individual spacer sequences.

Autor: Xue C; Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, IA 50011, USA., Seetharam AS; Genome Informatics Facility, Office of Biotechnology, Iowa State University Ames, IA 50011, USA., Musharova O; Institutes of Gene Biology and Molecular Genetics, Russian Academy of Sciences, Moscow 119991, Russia Skolkovo Institute of Science and Technology, Skolkovo, Russia, Moscow, Russia Peter the Great Polytechnical University, St. Petersburg, Russia., Severinov K; Institutes of Gene Biology and Molecular Genetics, Russian Academy of Sciences, Moscow 119991, Russia Skolkovo Institute of Science and Technology, Skolkovo, Russia, Moscow, Russia Peter the Great Polytechnical University, St. Petersburg, Russia Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Brouns SJ; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen, the Netherlands., Severin AJ; Genome Informatics Facility, Office of Biotechnology, Iowa State University Ames, IA 50011, USA., Sashital DG; Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, IA 50011, USA sashital@iastate.edu.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2015 Dec 15; Vol. 43 (22), pp. 10831-47. Date of Electronic Publication: 2015 Nov 19.

An updated evolutionary classification of CRISPR-Cas systems.

Autor: Makarova KS; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA., Wolf YI; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA., Alkhnbashi OS; Bioinformatics group, Department of Computer Science, University of Freiberg, Georges-Kohler-Allee 106, 79110 Freiberg, Germany., Costa F; Bioinformatics group, Department of Computer Science, University of Freiberg, Georges-Kohler-Allee 106, 79110 Freiberg, Germany., Shah SA; Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, DK2200 Copenhagen N, Denmark., Saunders SJ; Bioinformatics group, Department of Computer Science, University of Freiberg, Georges-Kohler-Allee 106, 79110 Freiberg, Germany., Barrangou R; Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina 27606, USA., Brouns SJ; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703HB Wageningen, Netherlands., Charpentier E; Department of Regulation in Infection Biology, Helmholtz Centre for Infection Research, D-38124 Braunschweig, Germany., Haft DH; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA., Horvath P; DuPont Nutrition and Health, BP10, Dangé-Saint-Romain 86220, France., Moineau S; Département de Biochimie, de Microbiologie et de Bio-informatique, Faculté des Sciences et de Génie, Groupe de Recherche en Écologie Buccale, Félix d'Hérelle Reference Center for Bacterial Viruses, Faculté de médecine dentaire, Université Laval, Québec City, Québec, Canada., Mojica FJ; Departamento de Fisiología, Genética y Microbiología. Universidad de Alicante. 03080-Alicante, Spain., Terns RM; Biochemistry and Molecular Biology, Genetics and Microbiology, University of Georgia, Davison Life Sciences Complex, Green Street, Athens, Georgia 30602, USA., Terns MP; Biochemistry and Molecular Biology, Genetics and Microbiology, University of Georgia, Davison Life Sciences Complex, Green Street, Athens, Georgia 30602, USA., White MF; Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, KY16 9TZ, UK., Yakunin AF; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, M5S 3E5, Canada., Garrett RA; Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, DK2200 Copenhagen N, Denmark., van der Oost J; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703HB Wageningen, Netherlands., Backofen R; Bioinformatics group, Department of Computer Science, University of Freiberg, Georges-Kohler-Allee 106, 79110 Freiberg, Germany.; BIOSS Centre for Biological Signaling Studies, Cluster of Excellence, University of Freiburg, Germany., Koonin EV; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA.

Publikováno v: Nature reviews. Microbiology [Nat Rev Microbiol] 2015 Nov; Vol. 13 (11), pp. 722-36. Date of Electronic Publication: 2015 Sep 28.

Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli.

Autor: Jackson RN; Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA., Golden SM; Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA., van Erp PB; Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA., Carter J; Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA., Westra ER; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands., Brouns SJ; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands., van der Oost J; Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands., Terwilliger TC; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA., Read RJ; Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, UK., Wiedenheft B; Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA. bwiedenheft@gmail.com.

Publikováno v: Science (New York, N.Y.) [Science] 2014 Sep 19; Vol. 345 (6203), pp. 1473-9. Date of Electronic Publication: 2014 Aug 07.