Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Brock Binkowski"'
Autor:
Julia K. Gilden, Pete Stecha, Aileen Paguio, Jim Hartnett, Brock Binkowski, Dan Lazar, Frank Fan, Mei Cong
Publikováno v:
Cancer Research. 83:3202-3202
Despite significant clinical advances in CAR-T cell therapies for cancer, the ability to precisely and reproducibly analyze the potency of these drug products remains a challenge. As new CAR-T products and production methodologies are developed, it i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a0f4dbf651b2133778d45507c3217fa4
https://doi.org/10.1158/1538-7445.am2023-3202
https://doi.org/10.1158/1538-7445.am2023-3202
Autor:
James D Vasta, Chad Zimprich, Q. Zheng, Kevan M. Shokat, Matthew B. Robers, D. M. Peacock, Cesear Corona, Ziyang Zhang, M. R. Thomas, J. A. Walker, M. T. Beck, Brock Binkowski
Current small molecule inhibitors of KRAS(G12C) bind irreversibly in the switch-II pocket, exploiting the strong nucleophilicity of the acquired cysteine as well as the preponderance of the GDP-bound form of this mutant. Nevertheless, many oncogenic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7c35124b797f8b5dadc1d8b3c26c7dcc
https://doi.org/10.1101/2021.10.15.464544
https://doi.org/10.1101/2021.10.15.464544
Autor:
Mei Cong, Maria J. Leandro, Patrick J. Duriez, Venkat Reddy, Brock Binkowski, Mark S. Cragg, H.T. Claude Chan, Robert J. Oldham, Geraldine Cambridge, Dun Li, Falk Nimmerjahn, Richard J. Stopforth, Philip G. Hargreaves, Alison L. Tutt, Anja Lux, Chad Zimprich
Publikováno v:
The Journal of Immunology. 200:1937-1950
Fc gamma receptors (FcγR) are involved in multiple aspects of immune cell regulation, are central to the success of monoclonal antibody (mAb) therapeutics and underpin the pathology of several autoimmune diseases. However, reliable assays capable of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a0099d71920f39c73f130dd25748fdf
https://doi.org/10.4049/jimmunol.1700832
https://doi.org/10.4049/jimmunol.1700832
Autor:
Brock Binkowski, Anton Shakhmin, Keith V. Wood, Joel R. Walker, Thomas Machleidt, Thomas A. Kirkland, Mary P. Hall
Publikováno v:
Chemistry - A European Journal. 22:10369-10375
The growing popularity of bioluminescent assays has highlighted the need for coelenterazine analogues possessing properties tuned for specific applications. However, the structural diversity of known coelenterazine analogues has been limited by curre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d848c2d8e4c008a1d724c59f58b610b9
https://doi.org/10.1002/chem.201601111
https://doi.org/10.1002/chem.201601111
Publikováno v:
Cancer Research. 79:3181-3181
Efforts to develop and commercialize T cell immunotherapies would benefit from sensitive and easy-to-use assays to monitor target cell killing. We have developed an approach to selectively measure the death of target cells mediated by CAR/TCR-T cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ba1b0df511024675de70604e855831db
https://doi.org/10.1158/1538-7445.am2019-3181
https://doi.org/10.1158/1538-7445.am2019-3181
Publikováno v:
Cancer Research. 79:2393-2393
Having a functional bioassay that is MOA-based, accurate, precise, robust and reproducible is critical for the development of antibody-based biologics. We have developed reporter bioassays that meet these criteria for a broad range of antibody modali
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::75c0cb7ebacf373a9158da207047d018
https://doi.org/10.1158/1538-7445.am2019-2393
https://doi.org/10.1158/1538-7445.am2019-2393
Autor:
Neesha Kara, Rachel Cooley, Mohamed Ismail, Keith V. Wood, Julian Downward, Brock Binkowski, Chloe Roustan, David C. Hancock, Ning Sze Hui, Roger George, Jonathan Tart
Publikováno v:
Wellcome Open Research
Targeting the interaction of proteins with weak binding affinities or low solubility represents a particular challenge for drug screening. The NanoLucâ ® Binary Technology (NanoBiTâ ®) was originally developed to detect protein-protein interactio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09fdd24698648de9ab69a73c70b01713
https://doi.org/10.12688/wellcomeopenres.15675.2
https://doi.org/10.12688/wellcomeopenres.15675.2
Autor:
Brock Binkowski, Marie K. Schwinn, Mary P. Hall, Lance P. Encell, Keith V. Wood, Robin Hurst, Andrew S. Dixon, Thomas Machleidt, Christopher T. Eggers, Kris Zimmerman
Publikováno v:
ACS chemical biology. 13(2)
Intracellular signaling pathways are mediated by changes in protein abundance and post-translational modifications. A common approach for investigating signaling mechanisms and the effects induced by synthetic compounds is through overexpression of r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3aec22f8ab7d64937178c70761fa7c17
https://pubmed.ncbi.nlm.nih.gov/28892606
https://pubmed.ncbi.nlm.nih.gov/28892606
Autor:
Keith V. Wood, Brock Binkowski, Frank Fan, Marie K. Schwinn, Robin Hurst, Thomas Machleidt, Christopher T. Eggers, Braeden L. Butler, Lance P. Encell, Mary P. Hall
Publikováno v:
Cancer Research. 77:4013-4013
Dysregulation of protein expression is a key mechanism of tumorigenesis. The most commonly used approach to monitor changes in expression is to perform SDS-PAGE, followed by immunoblotting, a labor-intensive process that requires high-quality antibod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::172def52922110c953c1fe582fbb354b
https://doi.org/10.1158/1538-7445.am2017-4013
https://doi.org/10.1158/1538-7445.am2017-4013
Autor:
Brock Binkowski, Mary P. Hall, Monika G. Wood, Kristopher Zimmerman, Paul Otto, Keith V. Wood, Gediminas Vidugiris, Dieter Klaubert, Hélène A Benink, Matthew B. Robers, James Unch, Lance P. Encell, Michael P. Valley, Frank Fan, Poncho Meisenheimer, Braeden L. Butler, Thomas Machleidt, Christopher T. Eggers, Michael R. Slater
Publikováno v:
ACS Chemical Biology
Bioluminescence methodologies have been extraordinarily useful due to their high sensitivity, broad dynamic range, and operational simplicity. These capabilities have been realized largely through incremental adaptations of native enzymes and substra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b14760e5049c02c7d69b1883d21b6ca
https://doi.org/10.1021/cb3002478
https://doi.org/10.1021/cb3002478